文献类型：期刊文献

英文题名：An Ammonium-Formate-Driven Trienzymatic Cascade for ω-Transaminase-Catalyzed (R)-Selective Amination

作者：Chen, Fei-Fei[1];Zhang, Yu-Hui[1];Zhang, Zhi-Jun[1];Liu, Lei[1];Wu, Jian-Ping[2];Xu, Jian-He[1];Zheng, Gao-Wei[1]

机构：[1]East China Univ Sci & Technol, Shanghai Collaborat Innovat Ctr Biomfg, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Zhejiang Univ, Inst Bioengn, Coll Chem & Biol Engn, Hangzhou 310027, Zhejiang, Peoples R China

年份：2019

卷号：84

期号：22

起止页码：14987

外文期刊名：JOURNAL OF ORGANIC CHEMISTRY

收录：;EI(收录号：20194607687887);WOS:【SCI-EXPANDED(收录号:WOS:000497259900061)】；

基金：This work was financially supported by the National Natural Science Foundation of China (Nos. 21536004, 21472045, 21878085, and 21871085), Natural Science Foundation of Shanghai (No. 18ZR1408400), and the Fundamental Research Funds for the Central Universities (No. 22221818014).

语种：英文

外文关键词：Amino acids - Amines - Amination

摘要：(R)-Amination mediated by (R)-specific omega-transaminases generally requires costly D-alanine in excess to obtain the desired chiral amines in high yield. Herein, a one-pot, trienzymatic cascade comprising an (R)-specific omega-transaminase, an amine dehydrogenase, and a formate dehydrogenase was developed for the economical and eco-friendly synthesis of (R)-chiral amines. Using inexpensive ammonium formate as the sole sacrificial agent, the established cascade system enabled efficient omega-transaminase-mediated (R)-amination of various ketones, with high conversions and excellent ee (>99%); water and CO2 were the only waste products.