文献类型：期刊文献

英文题名：Selective tracking of ovarian-cancer-specific -glutamyltranspeptidase using a ratiometric two-photon fluorescent probe

作者：Shi, Ben[1,2];Zhang, Zhenyu[1,2];Jin, Qingqing[3];Wang, Zhiqiang[4,5];Tang, Jie[1,2];Xu, Ge[1,2];Zhu, Tianli[1,2];Gong, Xueqing[4,5];Tang, Xinjing[3];Zhao, Chunchang[1,2]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Key Lab Adv Mat, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Chem & Mol Engn, Inst Fine Chem, Shanghai 200237, Peoples R China;[3]Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China;[4]East China Univ Sci & Technol, Sch Chem & Mol Engn, Ctr Computat Chem, Key Lab Adv Mat, 130 Meilong Rd, Shanghai 200237, Peoples R China;[5]East China Univ Sci & Technol, Sch Chem & Mol Engn, Res Inst Ind Catalysis, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2018

卷号：6

期号：45

起止页码：7439

外文期刊名：JOURNAL OF MATERIALS CHEMISTRY B

收录：;EI(收录号：20184806143259);WOS:【SCI-EXPANDED(收录号:WOS:000451073100011)】；

基金：We gratefully acknowledge the financial support from the National Science Foundation of China (grant number 21672062).

语种：英文

外文关键词：Aromatic hydrocarbons - Chromophores - Cells - Cytology - Photons - Fluorescence - Diseases - Energy transfer - Cancer cells

摘要：Real-time tracking of GGT enzymatic activity in human ovarian cancer cells is a reliable method for accurate prediction of cancer diagnosis and management. Here, we report the two-photon ratiometric tracking of GGT activity in cancer cells based on a probe with switchable Forster resonance energy transfer properties. In the absence of GGT, the designed probe showed two well-resolved emission bands at 461 and 610 nm, corresponding to the 7-hydroxycoumarin donor and BODIPY acceptor, respectively. In contrast, GGT catalyzed cascade reactions including cleavage of the -glutamyl group and subsequent aromatic hydrocarbon transfer from the S to N atom increased the distance between the two chromophores, thus decreasing the FRET efficiency, with the recovery of the donor fluorescence at 461 nm. By exploiting this enzyme-triggered ratiometric measurement, successful differentiation of ovarian cancer cells from normal cells with this probe was realized by two-photon fluorescence confocal microscopy.