文献类型：期刊文献

英文题名：Purification and characterization of a novel β-1,3-glucanase from Arca inflata and its immune-enhancing effects

作者：Li, Chunlei[1,2];Wen, Yao[1];He, Ying[3];Zhu, Jianhua[3];Yin, Xin[2];Yang, Jianing[3];Zhang, Lixin[2,4];Song, Liyan[3];Xia, Xuekui[2];Yu, Rongmin[1,3]

机构：[1]Jinan Univ, Biotechnol Inst Chinese Mat Med, 601 Huangpu Ave West, Guangzhou 510632, Guangdong, Peoples R China;[2]Qilu Univ Technol, Shandong Acad Sci, Biol Inst, Jinan 250013, Shandong, Peoples R China;[3]Jinan Univ, Coll Pharm, Dept Nat Med Chem, Guangzhou 510632, Guangdong, Peoples R China;[4]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2019

卷号：290

起止页码：1

外文期刊名：FOOD CHEMISTRY

收录：;EI(收录号：20191306690696);WOS:【SCI-EXPANDED(收录号:WOS:000464920800001)】；

基金：This research work was financially supported by National Natural Science Foundation of China (Nos. 81503303 and 81374015), Ocean and Fisheries Science and Technology Development Projects of Guangdong Province (No. A201501C04), Major Science and Technology Projects/Significant New Drugs Creation of Guangdong Province (No. 2013A022100032), the Natural Science Foundation from Shandong Province (Nos. ZR2017MH025, ZR2017ZB0206), and the Taishan Scholar Project from Shandong Province to Lixin Zhang. The authors thank Dr. Dongbo Yu of The University of Chicago, Illinois, USA, for assistance in proofreading our manuscript.

语种：英文

外文关键词：Arca inflata; Endo-beta-1,3-Glucanase; Biochemical characterization; Immune-enhancing activity; Glycoprotein

摘要：A novel beta-1,3-glucanase from Arca inflata was purified using chromatography methods. It was determined as a glycoprotein comprising 23.65% carbohydrate content with O-linked glycan and showed specific activity of 90.01 +/- 1.2 U/mg against laminarin. The optimal pH and temperature for the activity of the glucanase were 6.0 and 40 degrees C, respectively. The affinity parameter of the glucanase using laminarin was determined as K-d = 13.09 mu M. The activity of the glucanase was 27 +/- 2.6% enhanced by 2-mM Mn2+ ions and inhibited by 40-50% using 2-mM Zn2+, Cu2+, or Ba2+ ions. The glucanase showed an endo-type cleavage mode and hydrolyzed laminarin into glucoses, disaccharides, trioligosaccharides, and tetraoligosaccharides. Otherwise, the glucanase exhibited immune-enhancing effects via significantly increasing the phagocytic activity of macrophages and inducing the release of nitric oxide, tumor necrosis factor a, and interleukin-6 in RAW264.7 cells. It might be used as a bifunctional additive for the food industry.