文献类型：期刊文献

英文题名：Effects of ethylene glycol on the synthesis of ampicillin using immobilized penicillin G acylase

作者：Wei, DZ; Yang, L

机构：[1]E China Univ Sci & Technol, Inst Biochem, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2003

卷号：78

期号：4

起止页码：431

外文期刊名：JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000182055400009)】；

语种：英文

外文关键词：penicillin G acylase; ampicillin; ethylene glycol; enzymatic synthesis

摘要：The effects of organic cosolvents on the synthesis of ampicillin from phenylglycine methyl ester (PGME) and 6-amino penicillanic acid (6-APA) using immobilized Bacillus megaterium penicillin G acylase have been examined. Several cosolvents were tested for their influence on the enzyme in terms of enzyme stability and hydrophobicity. Among the cosolvents tested, ethylene glycol was found to increase the yield of ampicillin by 39-50%. The effects of ethylene glycol on the pK(a) of PGME, the hydrolysis of ampicillin and PGME, and synthetase/amidase and esterase/amidase ratios were also studied. Experimental data indicated that ethylene glycol inhibited more the hydrolysis of the ampicillin than the hydrolysis of the PGME and the synthetase/amidase ratio varied from 0.2 to 0.88 when the concentration (v/v) of the cosolvent increased from 0 to 40%. The enhancement of the synthesis yield was mainly caused by the reduction in the hydrolysis of acyl donor (PGME) and product (ampicillin) in the water-cosolvent system. (C) 2003 Society of Chemical Industry.