文献类型：期刊文献

英文题名：Two dimensional and three dimensional interactions between bovine serum albumin and chondroitin sulfate

作者：Xu, Shouhong[1]; Yonese, Masakatsu[2]

机构：[1]E China Univ Sci & Technol, Dept Chem, Shanghai 200237, Peoples R China;[2]Nagoya City Univ, Grad Sch Pharmaceut Sci, Mizuho Ku, Nagoya, Aichi 4678603, Japan

年份：2007

卷号：39

期号：4

起止页码：298

外文期刊名：POLYMER JOURNAL

收录：;EI(收录号：20072410649526);WOS:【SCI-EXPANDED(收录号:WOS:000246869200004)】；

语种：英文

外文关键词：Na(2)ChS; BSA; two dimensional interaction; three dimensional interaction; QCM

摘要：The interaction between glycosaminoglycan and protein in solution and at interface are important for the biofunctions of tissues. We previously reported that the binding behavior between sodium hyaluronate NaHA and bovine serum albumin BSA both in solution and at interface. Here, two- and three-dimensional interactions between sodium chondroitin sulfate Na(2)ChS and BSA were studied by an electrophoretic light scattering ELS and a quartz crystal microbalance QCM method. Na(2)ChS molecules bound to BSA molecules by an electrostatic force and form soluble complex in solution. Their molar ratio gamma = n(BSA)/n(ChS) of the saturated complex was about 6-7. However, the value of gamma was about 1 at interface when the binding between Na(2)ChS and BSA became saturated. Na(2)ChS molecules could adsorb onto a BSA monolayer by a hydrophobic force in a Langmuir type. Its adsorption layer was a double-layer structure from the results. The results of Na(2)ChS-BSA complex in solution and at interface were discussed by comparing with the binding behavior between sodium hyaluronate NaHA and BSA to elucidate the effects of the sulfate group and charge density of Na(2)ChS.