文献类型：期刊文献

英文题名：A polyethyleneimine-functionalized polymer substrate polar stationary phase

作者：Shen, Yufeng[1];Geng, Huiliang[1];Zhang, Feifang[1];Li, Zongying[1];Yang, Bingcheng[1]

机构：[1]East China Univ Sci & Technol, Engn Res Ctr Pharmaceut Proc Chem, Shanghai Frontiers Sci Ctr Optogenet Tech Cell Met, Sch Pharm, 130 Meilong RD, Shanghai 200237, Peoples R China

年份：2023

卷号：1689

外文期刊名：JOURNAL OF CHROMATOGRAPHY A

收录：;EI(收录号：20230113330053);WOS:【SCI-EXPANDED(收录号:WOS:000938081800001)】；

基金：Acknowledgments It was sponsored by National Natural Science Foundation of China (No. 21974044) .

语种：英文

外文关键词：Hydrophilic interaction liquid; chromatography; Polymer; Stationary phase; Polyethyleneimine; Bleed

摘要：A polyethyleneimine (PEI)-functionalized polymer substrate polar stationary phase was prepared for hy-drophilic interaction chromatography (HILIC) by grafting PEI onto poly(styrene-divinylbenzene) (PS-DVB) microspheres. The phase shows a U-shape retention profile and it exhibits typical hydrophilic character-istic when the organic solvent fraction in the mobile phase is > 60%. Hydrogen bonding, anion exchange, and hydrophobic interaction are involved in the retention mechanism. Good separation and unique selec-tivity for acidic, basic and neutral polar analytes were achieved. It showed extremely low column bleed (comparable to that of blank) under gradient elution mode (even to 50% fraction of water) and wide pH tollerance range (at least 1-13).(c) 2022 Elsevier B.V. All rights reserved.