文献类型：期刊文献

英文题名：Competitive immunoassay for clenbuterol using capillary electrophoresis with laser-induced fluorescence detection

作者：Zhou, Jieyu[1]; Xu, Xueshu[1]; Wang, Yuxiao[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2007

卷号：848

期号：2

起止页码：226

外文期刊名：JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

收录：;EI(收录号：20071310511434);WOS:【SCI-EXPANDED(收录号:WOS:000245762600007)】；

语种：英文

外文关键词：clenbuterol; capillary electrophoresis; immunoassay

摘要：A competitive immunoassay for detecting clenbuterol in urine was established by capillary electrophoresis (CE) with laser-induced fluorescence (LIF). The clenbuterol was conjugated with bovine serum albumin (BSA), and then the derivative was labeled with fluorescein isothiocyanate (FITC) and competes for antibody with free clenbuterol in the sample. Under the optimal conditions, Free and bound FITC labeled clenbuterol was separated within 8 min with the relative standard deviation (R.S.D.) 0.72% for migration time and 2.8% for peak area. The detection limit reached 0.7 ng/ml. (c) 2006 Elsevier B.V. All rights reserved.