文献类型：期刊文献

英文题名：Development of a large scale process for the conversion of polysialogangliosides to monosialotetrahexosylganglioside with a novel strain of Brevibacterium casei producing sialidase

作者：Peng, Yan-Feng[1]; Wang, Xue-Dong[1]; Wei, Dong-Zhi[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China

年份：2007

卷号：29

期号：6

起止页码：885

外文期刊名：Biotechnology Letters

收录：EI(收录号：20071810578824)

语种：英文

外文关键词：Bacteria - Bioconversion - Biotechnology - Brain - Cell culture - Enzymes

摘要：A bioconversion process of producing GM1 (monosialotetrahexosylganglioside) on an industrial scale was developed with a novel sialidase-producing strain Brevibacterium casei. The sialidase hydrolyzed polysialogangliosides to produce GM1 but did not act on GM1. When Brevibacterium casei was cultured in a synthetic medium containing crude pig brain gangliosides (10% w/v) at 30°C for 24 h in a 50 l fermenter, most of the polysialogangliosides were converted to GM1. The content of GM1 was increased from 9% in crude gangliosides to 45% with 70% (w/w) yield. ? 2007 Springer Science+Business Media B.V.