文献类型：期刊文献

英文题名：Cagelike mesoporous silica encapsulated with microcapsules for immobilized laccase and 2, 4-DCP degradation

作者：Yang, Junya[1];Huang, Yan[1];Yang, Yuxiang[1];Yuan, Hongming[2];Liu, Xiangnong[3]

机构：[1]E China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[2]Jilin Univ, Coll Chem, State Key Lab Inorgan Synth & Preparat Chem, Changchun 130012, Peoples R China;[3]Yangzhou Univ, Anal Test Ctr, Yangzhou 225009, Jiangsu, Peoples R China

年份：2015

卷号：38

起止页码：52

外文期刊名：JOURNAL OF ENVIRONMENTAL SCIENCES

收录：;EI(收录号：20160201773078);WOS:【SCI-EXPANDED(收录号:WOS:000370547800007)】；

基金：This work was supported by the National Natural Science Foundation of China (20577010, 20971043) and the Open Project Program of State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, Jilin University (2011-30).

语种：英文

外文关键词：Laccase-cMSS; mLbL-laccase; 2,4-Dichlorophenol; Degradation efficiency; Reusability; Degradation mechanism

摘要：In this study, cage-like mesoporous silica was used as the carrier to immobilize laccase by a physical approach, followed by encapsulating with chitosan/alginate microcapsule membranes to form microcapsules of immobilized laccase based on layer-by-layer technology. The relationship between laccase activity recovery/leakage rate and the coating thickness was simultaneously investigated. Because the microcapsule layers have a substantial network of pores, they act as semipermeable membranes, while the laccase immobilized inside the microcapsules acts as a processing plant for degradation of 2,4-dichlorophenol. The microcapsules of immobilized laccase were able to degrade 2,4-dichlorophenol within a wide range of 2,4-dichlorophenol concentration, temperature and pH, with mean degradation rate around 62%. Under the optimal conditions, the thermal stability and reusability of immobilized laccase were shown to be improved significantly, as the removal rate and degradation rate remained over 40.2% and 33.8% respectively after 6 cycles of operation. Using mass spectrometry (MS) and nuclear magnetic resonance (NMR), diisobutyl phthalate and dibutyl phthalate were identified as the products of 2,4-dichlorophenol degradation by the microcapsules of immobilized laccase and laccase immobilized by a physical approach, respectively, further demonstrating the degradation mechanism of 2,4-dichlorophenol by microcapsule-immobilized laccase. (C) 2015 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V.