文献类型：期刊文献

中文题名：壳聚糖季铵盐基杂化支架的制备及其作为rhBMP-2载体的研究

英文题名：Research of quaternized chitosan based hybrid scaffold as a delivery system for rhBMP-2

作者：胡园园[1];王靖[1];刘昌胜[1]

机构：[1]华东理工大学教育部医用生物材料工程研究中心,上海200237

年份：2014

卷号：45

期号：14

起止页码：14001

中文期刊名：功能材料

外文期刊名：Journal of Functional Materials

收录：CSTPCD;;EI(收录号：20143318074339);Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

基金：科技部国家重大科学研究计划资助项目(2012CB933600)

语种：中文

中文关键词：骨修复;壳聚糖;骨形态发生蛋白;磷酸钙

外文关键词：bone regeneration; chitosan; bone morphogenetic protein; calcium phosphate

摘要：采用环氧衍生物开环法制备壳聚糖季铵盐,通过其与甘油磷酸钠的分子间作用力交联成凝胶,冻干后得多孔支架,用于装载骨形态发生蛋白-2应用于骨修复领域;同时引入β-TCP作为物理交联点,在提高支架力学强度的同时,更好地调控支架的溶胀行为,延长rhBMP-2的释放时间,并且在异位诱导成骨的动物实验中取得良好效果。
Quaternized chitosan was synthesized by the reaction of chitosan and glycidyltrimethylammonium chloride （GTMAC）and named as N-[（2-hydroxy-3-trimethylammonium）propyl]chitosan chloride （HTCC）. A hydrogel system composed of HTCC andβ-glycerophosphate （β-GP）was obtained by intermolecular forces. Introduction ofβ-TCP granules improved the mechanical strength and controlled the swelling behavior and pro-longed the releasing period for rhBMP-2 of the hydrogel scaffold.Moreover,rhBMP-2 loaded scaffolds were as-sessed in ectopic bone formation.The results indicate that porous HTCC/β-GP/β-TCP/rhBMP-2 scaffolds are promising candidates for application in tissue engineering of bone.