文献类型：期刊文献

英文题名：3D Dynamic Culture of Rabbit Articular Chondrocytes Encapsulated in Alginate Gel Beads Using Spinner Flasks for Cartilage Tissue Regeneration

作者：Xu, Feiyue[1];Xu, Lei[1];Wang, Qi[1];Ye, Zhaoyang[1];Zhou, Yan[1];Tan, Wen-Song[1]

机构：[1]E China Univ Sci & Technol, Sch Bioengn, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2014

卷号：2014

外文期刊名：BIOMED RESEARCH INTERNATIONAL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000347321500001)】；

基金：This research was supported by National Special Fund for State Key Laboratory of Bioreactor Engineering (2060204), National Natural Science Foundation of China (31000424, 31170951), Specialized Research Fund for Doctoral Program of Higher Education (20100074120009), and SRF for ROCS (SEM).

语种：英文

摘要：Cell-based therapy using chondrocytes for cartilage repair suffers from chondrocyte dedifferentiation. In the present study, the effects of an integrated three-dimensional and dynamic culture on rabbit articular chondrocytes were investigated. Cells (passages 1 and 4) were encapsulated in alginate gel beads and cultured in spinner flasks in chondrogenic and chondrocyte growth media. Subcutaneous implantation of the cell-laden beads was performed to evaluate the ectopic chondrogenesis. It was found that cells remained viable after 35 days in the three-dimensional dynamic culture. Passage 1 cells demonstrated a proliferative growth in both media. Passage 4 cells showed a gradual reduction in DNA content in growth medium, which was attenuated in chondrogenic medium. Deposition of glycosaminoglycans (GAG) was found in all cultures. While passage 1 cells generally produced higher amounts of GAG than passage 4 cells, GAG/DNAbecame similar on day 35 for both cells in growth media. Interestingly, GAG/DNA in growth medium was greater than that in chondrogenic medium for both cells. Based on GAG quantification and gene expression analysis, encapsulated passage 1 cells cultured in growth medium displayed the best ectopic chondrogenesis. Taken together, the three-dimensional and dynamic culture for chondrocytes holds great potential in cartilage regeneration.