文献类型：期刊文献

英文题名：A novel open-tubular capillary electrochromatography with magnetic nanoparticle coating as stationary phase

作者：Zhu, Yaxian[1];Zhou, Chaoran[2];Qin, Sasa[1];Ren, Zhiyu[2];Zhang, Lingyi[1];Fu, Honggang[2];Zhang, Weibing[1]

机构：[1]E China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]Heilongjiang Univ, Key Lab Funct Inorgan Mat Chem, Minist Educ Peoples Republ China, Harbin, Peoples R China

年份：2012

卷号：33

期号：2

起止页码：340

外文期刊名：ELECTROPHORESIS

收录：;EI(收录号：20231613929274);WOS:【SCI-EXPANDED(收录号:WOS:000298849700010)】；

基金：The financial support from the 973 project from the Ministry of Science and Technology of People's Republic of China (No. 2011CB910403), the Key Program Projects of the National Natural Science Foundation of China (No. 21031001) and the National Natural Science Foundation of China (No. 21075039/20971040) are gratefully acknowledged.

语种：英文

外文关键词：CEC; Magnetic coating; Magnetic nanoparticles; Open-tubular column

摘要：A novel open-tubular capillary electrochromatography (OT-CEC) with modified core/shell magnetic nanoparticles coating as stationary phase was introduced using external magnetic force to fix magnetic nanoparticles. The magnetic nanoparticles coating inside the capillary columns could be easily regenerated by removing and re-applying the external magnetic field. Magnetic field intensity, concentration and flow rate of nanoparticles suspension were investigated to achieve simple and stable preparation. Mixture of five organic acids was used as the marker sample to evaluate the OT-CEC system, and the relative column efficiency of anthranilic acid reaches 220?000 plates/m. The excellent within-column and between-column repeatability has been testified with the RSDs of retention time of less than 1.51 and 5.29%, respectively. The aqueous extract of rhizoma gastrodiae was analyzed by the OT-CEC system, and 23 peaks were eluted in 30?min. Compared with conventional open-tubular capillary column, this new system shows faster separation speed and higher column efficiency from the larger surface area of nanoparticles. It has great potential in the method development for the analysis of complex samples, since magnetic coating can effectively prolong the column life by expediently replacing stationary phase to eliminate the pollution or irreversible adsorption.