文献类型：期刊文献

英文题名：Immobilized penicillin G acylase on mesoporous silica: The influence of pore size, pore volume and mesophases

作者：Lue, Yongjun[1];Guo, Yanglong[1];Wang, Yanqin[1];Liu, Xiaohui[1];Wang, Yunsong[1];Guo, Yun[1];Zhang, Zhigang[1];Lu, Guanzhong[1]

机构：[1]E China Univ Sci & Technol, Adv Mat Lab, Res Inst Ind Catalysis, Shanghai 200237, Peoples R China

年份：2008

卷号：114

期号：1-3

起止页码：507

外文期刊名：MICROPOROUS AND MESOPOROUS MATERIALS

收录：;EI(收录号：20082611336053);WOS:【SCI-EXPANDED(收录号:WOS:000258432100052)】；

语种：英文

外文关键词：penicillin G acylase (PGA); immobilization; mesoporous silica; pore size; mesophase

摘要：Penicillin G acylase (PGA) was immobilized on KIT-6 and SBA-15 with different pore sizes to study the influence of pore size, pore volume and mesophases on the immobilized enzyme (IME) activity. The results show that the pore size, pore volume, mesophase and surface area have,in obvious influence on the IME activity, of which the pore size is the most important factor. The activity of PGA immobilized on KIT-6/130 reaches to 3522 IU/g by dry support, which is higher than that ever reported for carrier-bound penicillin G acylase [A.I. Kallenberg, F. van Rantwijk, R.A. Sheldon, Adv. Synth. Catal. 347 (2005) 905-926]. Because of the covalent immobilization, the operational stability of IME increases. (C) 2007 Elsevier Inc. All rights reserved.