文献类型：期刊文献

英文题名：In situ-formed adhesive hyaluronic acid hydrogel with prolonged amnion-derived conditioned medium release for diabetic wound repair

作者：Zhang, Yiqing[1];Zheng, Yongjun[1,2];Shu, Futing[2];Zhou, Renjie[1];Bao, Bingkun[3];Xiao, Shichu[2];Li, Kai[1];Lin, Qiuning[3];Zhu, Linyong[1,3];Xia, Zhaofan[2]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[2]Naval Med Univ, Changhai Hosp, Dept Burn Surg, Shanghai 200433, Peoples R China;[3]Shanghai Jiao Tong Univ, Sch Biomed Engn, Shanghai 200240, Peoples R China

年份：2022

卷号：276

外文期刊名：CARBOHYDRATE POLYMERS

收录：;EI(收录号：20214411091515);WOS:【SCI-EXPANDED(收录号:WOS:000720832600002)】；

基金：This work was supported by National Key Research and Development Program of China (2019YFA0110500) ; the National Natural Science Foundation of China (82072170, 81701905, 81930057, 81772076, 81871559) ; Youth Incubation Plan of the Military Medical Science and Technology (20QNPY035) ; CAMS Innovation Fund for Medical Sciences (2019-I2M-5-076) . We would like to thank Editage ( www.editage.cn) for English language editing.

语种：英文

外文关键词：Hydrogel; Photo-crosslinking; Amnion-derived conditioned medium; Diabetic ulcer; Wound repair

摘要：Hydrogels have long been used for encapsulating stem cell-derived conditioned mediums to achieve skin regeneration after wounding. However, inappropriate mechanical strength, low adhesion and low elasticity limit their clinical application. To address these challenges, we engineered a hyaluronic acid-based hydrogel grafted with methacrylic anhydride and N-(2-aminoethyl)-4-[4-(hydroxymethyl)-2-methoxy-5-nitrophenoxy]-butanamide (NB) groups to encapsulate a lyophilized amnion-derived conditioned medium (AM-CM). This hydrogel can photopolymerize in situ within 3 s by photo-initiated free-radical crosslinking between methacrylate moieties. Meanwhile, the formed o-nitrosobenzaldehyde groups by photo-irradiation could covalently bond with the amino groups of tissue surface, which allowed strong tissue adhesion. Furthermore, the hydrogel possessed excellent mechanical properties, high elasticity, favorable biocompatibility and prolonged AM-CM release. Our further vitro and in vivo studies showed that the hydrogel significantly accelerated diabetic wound healing by regulating macrophage polarization and promoting angiogenesis. The engineered hydrogel with AM-CM release has high potential to treat chronic wounds in clinics.