文献类型：期刊文献

中文题名：珍珠角壳蛋白中氨基酸的高效液相色谱分析

英文题名：Determination of Amino Acids in Protein of Pearls by Reversed-phase High Performance Liquid Chromatography

作者：金凤明[1];冯学伟[1];李桂贞[1]

机构：[1]华东理工大学分析测试中心,上海200237

年份：2001

卷号：27

期号：1

起止页码：103

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：珍珠;氨基酸;异硫氰酸苯酯;反相高效液相色谱;角壳蛋白;组分;含量测定;C18反相柱

外文关键词：pearls; protein; amino acids; phenylisothiocyanate; reverse phase high performance liquid chromatography (RP HPLC)

摘要：采用国产 C1 8反相柱 ,对以异硫氰酸苯酯 (PITC)为柱前衍生化试剂的高效液相色谱测定氨基酸的方法进行了研究。在室温下 ,以 p H=6.3的 HAc- Na Ac缓冲溶液及乙腈水溶液为流动相进行梯度洗脱 ,1 8种常见氨基酸在 30 min内获得了较好的分离 ,并且测定了珍珠角壳蛋白的氨基酸组成 ,结果表明 :珍珠角壳蛋白主要由天冬氨酸、甘氨酸、丝氨酸、丙氨酸、亮氨酸等 1 0种氨基酸组成 ,这些氨基酸约占蛋白总量的 85%。
By use of octadecyl (C18) column made in China, a RP HPLC method was studied using phenylisothiocyanate (PITC) as the pre column derivatizing reagent for the determination of amino acids. Under room temperature, pH6.3 HAc NaAc buffers, acetonitrile water solutions as the mobile phase and the use of the gradient elution mode, fine resolution of phenylthiocarbamyl derivatives of 18 kinds of common amino acids was achieved after about 30 minutes and the content of amino acids (except Trp) in the hydrolysate of protein of pearl was determined. The results showed that the proteins in pearls mainly consist of 10 kinds of amino acids such as Asp, Glu, Ser, Gly, Ala, Leu and so on which account for about 85 percent of total amount of protein.