文献类型：期刊文献

英文题名：Reconstruction and analysis of a genome-scale metabolic model of the vitamin C producing industrial strain Ketogulonicigenium vulgare WSH-001

作者：Zou, Wei[1,3];Liu, Liming[1,2,3];Zhang, Jing[4];Yang, Haoru[1,3];Zhou, Maoda[1,3];Hua, Qiang[2];Chen, Jian[1,3]

机构：[1]Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi 214122, Peoples R China;[2]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[3]Jiangnan Univ, Minist Educ, Key Lab Ind Biotechnol, Wuxi 214122, Jiangsu, Peoples R China;[4]Sichuan Univ Sci & Engn, Coll Bioengn, Zigong 643000, Sichuan, Peoples R China

年份：2012

卷号：161

期号：1

起止页码：42

外文期刊名：JOURNAL OF BIOTECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000306662800006)】；

基金：The author would like to thank Dr. Jens Nielsen, Professor at Chalmers University of Technology, for technological help on the model reconstruction. This work was supported by grants from the Key Program of National Natural Science Foundation of China (20836003), Program for New Century Excellent Talents in University (NCET-10-0456), the Priority Academic Program Development of Jiangsu Higher Education Institutions, Program for Advanced Talents within Six Industries of Jiangsu Province (2011-NY033), the Open Funding Project of the State Key Laboratory of Bioreactor Engineering, the 111 Project (111-2-06) and the Fundamental Research Funds for the Central Universities (JUDCF10017).

语种：英文

外文关键词：Genome-scale metabolic model; Ketogulonicigenium vulgare; 2-keto-L-Gulonic acid; Sulfate metabolism; Vitamin C

摘要：Ketogulonicigenium vulgare WSH001 is an industrial strain commonly used in the vitamin C producing industry. In order to acquire a comprehensive understanding of its physiological characteristics, a genome-scale metabolic model of K. vulgare WSH001, iWZ663, including 830 reactions, 649 metabolites, and 663 genes, was reconstructed by genome annotation and literature mining. This model was capable of predicting quantitatively the growth of K. vulgare under l-sorbose fermentation conditions and the results agreed well with experimental data. Furthermore, phenotypic features, such as the defect in sulfate metabolism hampering the syntheses of l-cysteine, l-methionine, coenzyme A (CoA), and glutathione, were investigated and provided an explanation for the poor growth of K. vulgare in monoculture. The model presented here provides a validated platform that can be used to understand and manipulate the phenotype of K. vulgare to further improve 2-KLG production efficiency. (C) 2012 Elsevier B.V. All rights reserved.