文献类型：期刊文献

中文题名：Increasing glycolytic flux in Torulopsis glabrata by redirecting ATP production from oxidative phosphorylation to substrate-level phosphorylation

英文题名：Increasing glycolytic flux in Torulopsis glabrata by redirecting ATP production from oxidative phosphorylation to substrate-level phosphorylation

作者：Liu, LM[1,2,3];Li, Y[1,2,3];Du, GC[1,2,3];Chen, J[1,2,3]

机构：[1]So Yangtze Univ, Sch Biotechnol, Wuxi 214036, Peoples R China;[2]So Yangtze Univ, Sch Biotechnol, Minist Educ, Key Lab lnd Biotechnol, Wuxi, Peoples R China;[3]E China Univ Sci ＆ Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China

年份：2007

卷号：21

期号：11

起止页码：11

中文期刊名：中国生物学文摘

外文期刊名：Chinese Biological Abstracts

语种：英文

中文关键词：糖酵解通量;隐球菌;ATP;氧化磷酸化;底物磷酸化

外文关键词：F0F1-ATPase; glycolytic flux; neomycin-resistant mutant; oligomycin; Torulopsis glabrataMETABOLIC-CONTROLANALYSIS; SACCHAROMYCES-CEREVISIAE; ESCHERICHIA-COLI; ADENOSINE-TRIPHOSPHATASE; PYRUVATE PRODUCTION; RESPIRATORY CONTROL; FOF1 ATPASE; YEAST;IDENTIFICATION; MUTANTS

摘要：Aims： This study aimed at further increasing the pyruvate productivity of a multi-vitamin auxotrophic yeast Torulopsis glabrata by redirecting ATP production from oxidative phosphorylation to substrate-level phosphorylation. Methods and Results： We examined two strategies to decrease the activity of FOF1-ATPase. The strategies were to inhibit FOF1-ATPase activity by addition of 9 oligomycin, or to disrupt FOF1-ATPase by screening neomycin-resistant mutant. The addition of 0（.）05 inmol 1（-1） oligomycin to the culture broth of T. galabrata CCTCC M202019 resulted in a significantly decreased intracellular ATP level （35（.）7%） and a significantly increased glucose consumption rate （49（.）7%）. A neomycin-resistant mutant N07 was screened and selected after nitrosoguanidine mutagenesis of the parent strain T. glabrata CCTCC M202019. Compared with the parent strain,the FOFI-ATPase activity of the mutant N07 decreased about 65%. As a consequence, intracellular ATP level of the mutant N07 decreased by 24%, which resulted in a decreased growth rate and growth yield. As expected, glucose consumption rate and pyruvate productivity of the mutant N07 increased by 34% and 42.9%, respectively. Consistently, the activities of key glycolytic enzymes of the mutant N07, including phosphofructokinase, pyruvate kinase and glyceraldyde-3-phosphate dehydrogenase, increased by 63.7%, 28.8% and 14.4%, respectively. In addition, activi- ties of the key enzymes involved in electron transfer chain of the mutant N07 also increased. Conclusions： Impaired oxidative phosphorylation in T. glabrata leads to a decreased intracellular ATP production, thereby increasing the glycolytic flux. Significance and Impact of the Study.