文献类型：期刊文献

英文题名：Controllable manipulation of alginate-gelatin core-shell microcarriers for HUMSCs expansion

作者：Wu, Yanfei[1];Zheng, Yiling[1];Jin, Ziyang[2];Li, Shihao[2];Wu, Weiqian[1];An, Chenjing[1];Guo, Jiahao[1];Zhu, Zhihua[1];Zhou, Tian[3];Zhou, Yan[2];Cen, Lian[1]

机构：[1]East China Univ Sci & Technol, Sch Chem Engn, Dept Prod Engn, Shanghai Key Lab Multiphase Mat Chem Engn, 130 Mei Long Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Bioengn, State Key Lab Bioreactor Engn, 130 Mei Long Rd, Shanghai 200237, Peoples R China;[3]Shanghai Jiao Tong Univ, Sch Med, Shanghai Peoples Hosp 9, Coll Stomatol,Natl Ctr Stomatol,Natl Clin Res Ctr, Shanghai 200011, Peoples R China

年份：2022

卷号：216

起止页码：1

外文期刊名：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

收录：;EI(收录号：20240815594686);WOS:【SCI-EXPANDED(收录号:WOS:000861280500001)】；

语种：英文

外文关键词：Microcarrier; Cell expansion; HUMSCs; Microfluidic; Mechanical strength

摘要：Human umbilical cord mesenchymal stem cells (HUMSCs) are one of the most attractive sources of stem cells, and it is meaningful to design and develop a type of microcarriers with suitable mechanical strength for HUMSCs proliferation in order to acquire enough cells for cell-based therapy. Alginate-gelatin core-shell (AG) soft microcarriers were thus fabricated via a microfluidic device with droplet shearing/gelation facilities and surface coating for in vitro expansion of HUMSCs. The attachment and proliferation of HUMSCs on AG microcarriers with different mechanical strengths modulated by gelatin coating was studied, and the harvested cells were characterized to verity their differentiation potential. The obtained core-shell microcarriers were all uniform in size with a high mono-dispersity (CV < 5 %). An increase in the gelatin surface coating concentration from 0.5 % to 1.5 % would lead to the reduction in both the particle size of the microcarriers and swelling ratio upon the contact of culture medium, but increased elastic modulus. Microcarriers of 245.12 mu m with a gelatin coating elastic modulus of 27.5 kPa (AG10) were found to be the optimal substrate for HUMSCs with an initial attachment efficiency of 44.41 % and a 5-day expansion efficiency of 647 %. The cells harvested from AG10 still reserved their outstanding pluripotency. Fresh AG10 could smoothly transfer cells from a running microcarrier-cell system of confluence to serve as a convenient way of scaling-up the existing culture. The current study thus developed suitable microcarriers, AG10, for in vitro HUMSCs expansion with well reserve of cell multipotency, and also provided a manufacturing and surface manipulating strategy of precise production and fine regulation of microcarrier properties.