文献类型：期刊文献

英文题名：Hyaluronan Immobilized Polyurethane as a Blood Contacting Material

作者：Gong, Feirong[1];Lu, Yue[1];Guo, Hui[1];Cheng, Shujun[1];Gao, Yun[1]

机构：[1]E China Univ Sci & Technol, Sch Mat Sci & Engn, Key Lab Ultrafine Mat, Minist Educ, Shanghai 200237, Peoples R China

年份：2010

卷号：2010

外文期刊名：INTERNATIONAL JOURNAL OF POLYMER SCIENCE

收录：;EI(收录号：20214511126334);WOS:【SCI-EXPANDED(收录号:WOS:000208425400024)】；

基金：This paper was supported by the 863 Project of 2007AA02Z450 from the Ministry of Science and Technology of China. The authors thank Shanghai Aosi Biological Technology Service Co., Ltd. for the help in cell culture and coagulation time test.

语种：英文

外文关键词：Endothelial cells - Hyaluronic acid - Fourier transform infrared spectroscopy - Infrared reflection - Blood - Scanning electron microscopy

摘要：Hyaluronan (hyaluronic acid, HA) was immobilized onto the surface of amino-functionalized polyurethane films with the goal of obtaining a novel kind of biomaterial which had the potential in blood-contacting applications. The amino-functionalized polyurethane was prepared by synthesized acidic polyurethane whose pendant carboxyl groups were treated with an excess amount of 1,3-diaminopropane in the presence of N,N-carbonyldiimidazole (CDI). Attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), Raman spectroscopy (RS), scanning electron microscopy (SEM), and water contact angle measurement were used to confirm the surface changes at each step of treatment, both in morphologies and chemical compositions. APTT and PT results showed that HA immobilization could prolong the blood coagulation time, thus HA-immobilized polyurethane (PU-HA) exhibited improved blood compatibility. Cytotoxicity analysis showed that the PU-HA films synthesized in this study were cytocompatible and could support human vein endothelial cells (HUVECs) adhesion and proliferation.