文献类型：期刊文献

英文题名：Efficient extraction of intracellular reduced glutathione from fermentation broth of Saccharomyces cerevisiae by ethanol

作者：Xiong, Zhi-Qiang[1];Guo, Mei-Jin[1];Guo, Yuan-Xin[1];Chu, Ju[1];Zhuang, Ying-Ping[1];Zhang, Si-Liang[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2009

卷号：100

期号：2

起止页码：1011

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000261080700079)】；

基金：We thank Professor lain Hunter (University of Strathclyde, UK) for critical reading of the manuscript. We are also grateful to the Zhengshen Biotechnical Co., Ltd. (Gansu Province, PR China) for financial support and their industrial strain for GSH production. This work was supported by the Key Program of Shanghai Municipal Science and Technology Commission (No. 074958025 and No. 07DZ22914) and by the Shanghai Leading Academic Discipline Project (B505).

语种：英文

外文关键词：Reduced glutathione; Full factorial designs; Saccharomyces cerevisiae; Ethanol extraction

摘要：Reduced glutathione (GSH) from fermentation broth of Saccharomyces cerevisiae was extracted with ethanol without disruption of the cells. The effects of ethanol concentration, extraction temperature and extraction time were assessed by using 2(3) full factorial designs (FFD). Preliminary studies showed that ethanol concentration had the most influence on GSH yield by ethanol extraction, based on the first order regression coefficients derived using MINITAB software, and an optimal ethanol concentration (25%, v/v) was obtained. However, compared to the conventional extraction technique (hot water extraction), there was no significant advantage in yield of GSH from yeast cells using ethanol extraction under these optimized conditions. But ethanol extraction has several advantages, Such as lower energy consumption and lower protein concentration of extraction broth, which may reduce the complexity and cost of the purification process. Hence, ethanol extraction which does not disrupt yeast cells could be an inexpensive, simple and efficient alternative to conventional extraction techniques in the GSH industry. (c) 2008 Elsevier Ltd. All rights reserved.