文献类型：期刊文献

英文题名：Unveiling the mechanism of efficient β-phenylethyl alcohol conversion in wild-type Saccharomyces cerevisiae WY319 through multi-omics analysis

作者：Yang, Chenghan[1];Ren, Yilin[1];Ge, Lihao[1];Xu, Wenting[1];Hang, Haifeng[1];Mohsin, Ali[1];Tian, Xiwei[1];Chu, Ju[1];Zhuang, Yingping[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, POB 329,130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2024

卷号：19

期号：4

外文期刊名：BIOTECHNOLOGY JOURNAL

收录：;EI(收录号：20241515854338);WOS:【SCI-EXPANDED(收录号:WOS:001197587300001)】；

基金： National Key Research and Development Program of China, Grant/Award Number: 2021YFC2100203

语种：英文

外文关键词：high-yield mechanism; high-yielding strain; multi-omics; respiratory metabolism; Saccharomyces cerevisiae; beta-phenylethanol

摘要：beta-Phenylethanol (2-PE), as an important flavor component in wine, is widely used in the fields of flavor chemistry and food health. 2-PE can be sustainably produced through Saccharomyces cerevisiae. Although significant progress has been made in obtaining high-yield strains, as well as improving the synthesis pathways of 2-PE, there still lies a gap between these two fields to unpin. In this study, the macroscopic metabolic characteristics of high-yield and low-yield 2-PE strains were systematically compared and analyzed. The results indicated that the production potential of the high-yield strain might be contributed to the enhancement of respiratory metabolism and the high tolerance to 2-PE. Furthermore, this hypothesis was confirmed through comparative genomics. Meanwhile, transcriptome analysis at key specific growth rates revealed that the collective upregulation of mitochondrial functional gene clusters plays a more prominent role in the production process of 2-PE. Finally, findings from untargeted metabolomics suggested that by enhancing respiratory metabolism and reducing the Crabtree effect, the accumulation of metabolites resisting high 2-PE stress was observed, such as intracellular amino acids and purines. Hence, this strategy provided a richer supply of precursors and cofactors, effectively promoting the synthesis of 2-PE. In short, this study provides a bridge for studying the metabolic mechanism of high-yield 2-PE strains with the subsequent targeted strengthening of relevant synthetic pathways. It also provides insights for the synthesis of nonalcoholic products in S. cerevisiae.