文献类型：会议论文

英文题名：Molecular Cloning and Characterization of Glycerol Dehydrogenase from Klebsiella pneumoniae

作者：Liu, Yanhua[1];Zhao, Li[3];Zhang, Jianguo[4];Zheng, Yu[2]

机构：[1]Tianjin Univ Sci & Technol, Coll Sci, Tianjin 300457, Peoples R China;[2]Minist Educ, Key Lab Ind Fermentat Microbiol, Tianjin 300457, Peoples R China;[3]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[4]Univ Shanghai Sci & Technol, Sch Med Instrument & Food Engn, Shanghai 200093, Peoples R China

会议论文集：2nd International Conference on Applied Biotechnology (ICAB)

会议日期：NOV 28-30, 2014

会议地点：Tianjin, PEOPLES R CHINA

语种：英文

外文关键词：1,3-Propanediol; 1,3-Propanediol oxidoreductase; Glycerol dehydratase; Glycerol dehydrogenase; Klebsiella pneumonia

摘要：Glycerol dehydrogenase (GDH) was a key enzyme for 1,3-propanediol fermentation from glycerol. In this report, gene gdh, encoding GDH of Klebsiella pneumoniae KG1 was gained by PCR with genomic DNA as template. The open reading frame (ORF) of gdh consisted of 1,143 bp and encoded 380 amino acids with a deduced molecular mass of 42 kDa. The gene gdh was overexpressed in E. coli BL21 (DE3) and produced 886-fold higher activity of GDH than that of KG1. The optimum temperature of recombinant GDH was 37 degrees C and glycerol was the optimum substrate.