文献类型：期刊文献

英文题名：In Situ Strain-Level Detection and Identification of Vibrio parahaemolyticus Using Surface-Enhanced Raman Spectroscopy

作者：Xu, Jiajie[1,4];Turner, Jeffrey W.[2];Idso, Matthew[1];Biryukov, Stanley V.[2];Rognstad, Laurel[5];Gong, Heng[4];Trainer, Vera L.[2];Wells, Mark L.[3];Strom, Mark S.[2];Yu, Qiuming[1]

机构：[1]Univ Washington, Dept Chem Engn, Seattle, WA 98195 USA;[2]NOAA, Natl Marine Fisheries Serv, NW Fisheries Sci Ctr, Seattle, WA 98112 USA;[3]Univ Maine, Sch Marine Sci, Orono, ME 04469 USA;[4]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[5]Tennessee Technol Univ, Dept Chem Engn, Cookeville, TN 38505 USA

年份：2013

卷号：85

期号：5

起止页码：2630

外文期刊名：ANALYTICAL CHEMISTRY

收录：;EI(收录号：20131116106884);WOS:【SCI-EXPANDED(收录号:WOS:000317031600014)】；

基金：This work was supported in part by the University of Washington (UW) faculty start-up funds, the National Science Foundation (NSF CBET 1158609), and the West Coast Center for Oceans and Human Health, the NOAA Oceans and Human Health Initiative, and National Marine Fisheries Service. J.J.X. acknowledges a fellowship from the China Scholarship Council. Nanofabrication and studies of SERS were performed at the Nanotech User Facility, the UW site of the National Nanotechnology Infrastructure Network (NNIN) supported by the NSF. L.R. acknowledges the NNIN Research Experience for Undergraduates (REU) program supported by the NSF.

语种：英文

外文关键词：Finite difference time domain method - Light transmission - Substrates - Plasmonics - Bacteria - Raman spectroscopy

摘要：The outer membrane of a bacterium is composed of chemical and biological components that carry specific molecular information related to strains, growth stages, expressions to stimulation, and maybe even geographic differences. In this work, we demonstrate that the biochemical information embedded in the outer membrane can be used for rapid detection and identification of pathogenic bacteria using surface-enhanced Raman spectroscopy (SERS). We used seven different strains of the marine pathogen Vibrio parahaemolyticus as a model system. The strains represent four genetically distinct clades isolated from clinical and environmental sources in Washington, USA The unique quasi-3D (Q3D) plasmonic nanostructure arrays, optimized using finite-difference time-domain (FDTD) calculations, were used as SERS-active substrates for sensitive and reproducible detection of these bacteria. SERS barcodes were generated on the basis of SERS spectra and were used to successfully detect individual strains in both blind samples and mixtures. The sensing and detection methods developed in this work could have broad applications in the areas of environmental monitoring, biomedical diagnostics, and homeland security.