文献类型：期刊文献

中文题名：Interaction of DNA with Cationic Gemini Surfactant Trimethylene-1, 3-bis （dodecyldimethyl-ammonium bromide） and Anionic Surfactant SDS Mixed System

英文题名：Interaction of DNA with Cationic Gemini Surfactant Trimethylene-1, 3-bis (dodecyldimethyl-ammonium bromide) and Anionic Surfactant SDS Mixed System

作者：Zhao Xiaofang[1,2];Shang Yazhuo[1,2];Liu Honglai[1,2];Hu Ying[1,2];Jiang Jianwen[3]

机构：[1]E China Univ Sci & Technol, Adv Mat Lab, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Dept Chem, Shanghai 200237, Peoples R China;[3]Natl Univ Singapore, Dept Chem & Biomol Engn, Singapore 117576, Singapore

年份：2008

卷号：16

期号：6

起止页码：923

中文期刊名：Chinese Journal of Chemical Engineering

外文期刊名：CHINESE JOURNAL OF CHEMICAL ENGINEERING

收录：CSTPCD;;EI(收录号：20090211851310);Scopus;WOS:【SCI-EXPANDED(收录号:WOS:000262274100017)】；CSCD:【CSCD2011_2012】；

基金：Supported by the National Natural Science Foundation of China (20706013, 20736002), Program for Changjiang Scholars and Innovative Research Team in University (IRT0721), the III Project (1308021) and National University of Singapore.

语种：英文

中文关键词：interaction; DNA; gemini surfactant; SDS; conformation

外文关键词：interaction; DNA; gemini surfactant; SDS; conformation

摘要：The interaction of DNA with cationic gemini suffactant trimethylene-1,3-bis （dodecyl dimethyl-ammonium bromide） （12-3-12） and anionic surfactant sodium dodecyl sulfate （SDS） mixed system has been investigated by measuring the fluorescence, zeta potential, UV-Vis spectrum, and circular dichroism. In the absence of SDS, owing to the electrostatic and hydrophobic interactions, 12-3-12 forms micelle-like structure on the DNA chain before the micellization in bulk phase. For the mixed system of 12-3-12 and SDS, the negative charges on SDS can compete against DNA to bind with cationic 12-3-12 because of the stronger interaction between oppositely charged surfactants, and thus, the catanionic mixed micelles are formed before the formation of DNA/12-3-12 complexes. There-after, the positive charges on the mixed micelles bind with DNA, and thus, the change of the zeta potential from negative to positive is distinctly different from the system without SDS. Meanwhile, the existence of SDS postpones the exclusion of ethidium bromide （EB） from DNA/EB complexes. The conformation of DNA undergoes a change from native B-form to chiral ψ-phase as binding with 12-3-12 process. Upon adding SDS to the DNA/12-3-12 complex solution, however, DNA is released to the bulk and the ψ-phase returns to B-form again.
The interaction of DNA with cationic gemini surfactant trimethylene-1,3-bis (dodecyl dimethyl-ammonium bromide) (12-3-12) and anionic surfactant sodium dodecyl sulfate (SDS) mixed system has been investigated by measuring the fluorescence, zeta potential, UV-Vis spectrum, and circular dichroism. In the absence of SDS, owing to the electrostatic and hydrophobic interactions, 12-3-12 forms micelle-like structure on the DNA chain before the micellization in bulk phase. For the mixed system of 12-3-12 and SDS, the negative charges on SDS can compete against DNA to bind with cationic 12-3-12 because of the stronger interaction between oppositely charged surfactants. and thus, the catanionic mixed micelles are formed before the formation of DNA/12-3-12 complexes. Thereafter, the positive charges on the mixed micelles bind with DNA, and thus, the change of the zeta potential from negative to positive is distinctly different from the system without SDS. Meanwhile, the existence of SDS postpones the exclusion of ethidium bromide (EB) from DNA/EB complexes. The conformation of DNA undergoes a change from native B-form to chiral psi-phase as binding with 12-3-12 process. Upon adding SDS to the DNA/12-3-12 complex solution, however, DNA is released to the bulk and the y-phase returns to B-form again.