文献类型：期刊文献

英文题名：Medium optimization based on comparative metabolomic analysis of chicken embryo fibroblast DF-1 cells

作者：Lin, Jia[1];Yi, Xiaoping[1];Zhuang, Yingping[1]

机构：[1]East China Univ Sci & Technol, Sch Bioengn, State Key Lab Bioreactor Engn, 130 Mei Long Rd, Shanghai 200237, Peoples R China

年份：2019

卷号：9

期号：47

起止页码：27369

外文期刊名：RSC ADVANCES

收录：;EI(收录号：20193707423745);WOS:【SCI-EXPANDED(收录号:WOS:000487054800026)】；

基金：This study was funded by the Chinese National Programs for High Technology Research and Development (2015AA020801).

语种：英文

外文关键词：Animals - Cell culture - Metabolites - Viruses - Cell proliferation - Disease control

摘要：Chicken embryo fibroblast DF-1 cells are increasingly being used in the production of avian virus vaccines. However, the relatively low proliferative capacity does not meet the requirements of industrial production. In this study, we attempted to improve the proliferative capacity of DF-1 cells. The results of intracellular metabolomics showed that 28 types of metabolites could play roles in DF-1 cell growth based on the variance and timing analysis of intracellular metabolites from DF-1 cells grown in two media with distinct growth difference, DMEM/F12 (1 : 1) and DMEM. By examining the differences in the components in the two media, DOE was used to screen and optimize the growth medium for DF-1 cells. The maximum cell density was 40.72% higher, and the infectious bursal disease virus (IBDV) titer was 2.68 times higher, in the optimized medium than in the control. This study proposes a complete solution from metabolomics to media optimization.