文献类型：期刊文献

中文题名：酒石酸亚铁-标准曲线法检测绿茶提取物中茶多酚含量

英文题名：Determination of tea polyphenols in green tea extracts by ferrous tartrate-standard curve method

作者：邓祥[1];韩伟[1]

机构：[1]华东理工大学药学院,制药工程与过程化学教育部工程研究中心,上海市新药设计重点实验室,上海200237

年份：2020

卷号：42

期号：5

起止页码：677

中文期刊名：南京工业大学学报（自然科学版）

外文期刊名：Journal of Nanjing Tech University(Natural Science Edition)

收录：CSTPCD;;北大核心:【北大核心2017】；

语种：中文

中文关键词：茶多酚;酒石酸亚铁;方法学验证;福林酚比色法;标准曲线法

外文关键词：tea polyphenols;ferrous tartrate;methodological verification;Folin-Ciocalteu chromogenic method;standard curve method

摘要：采用酒石酸亚铁-标准曲线法检测茶叶提取物中茶多酚含量,单因素实验优化的显色条件为室温、蒸馏水4 mL、酒石酸亚铁溶液5 mL、以pH=7.5的磷酸盐缓冲液定容,该方法可即时检测。通过方法学验证实验,比较紫外分光光度法、酒石酸亚铁-标准曲线法及福林酚比色法测定茶多酚含量的适用性。结果表明:酒石酸亚铁-标准曲线法是一种高效、快捷的茶多酚含量检测方法,其适用范围广、受干扰程度小、结果可信度高。
The content of tea polyphenols in tea extracts was determined by ferrous tartrate-standard curve method.The color reaction conditions optimized by single factor experiments were as follows:room temperature,distilled water of 4 mL,ferrous tartrate solution of 5 mL,the volume was determined by phosphate buffer of pH=7.5,and this method can be detected immediately.The applicability of determination of tea polyphenols by the ultraviolet(UV)spectrophotometry,ferrous tartrate-standard curve method and Folin-Ciocalteu chromogenic method was compared by methodological verification experiments.Results showed that ferrous tartrate-standard curve method was an efficient and fast determination method for tea polyphenols,which had a wide range of application,a small degree of interference,and high credibility of the results.