文献类型：期刊文献

英文题名：Anaerobic Degradation of Paraffins by Thermophilic Actinobacteria under Methanogenic Conditions

作者：Liu, Yi-Fan[1,2,3,4];Chen, Jing[1,2,3];Liu, Zhong-Lin[1,2,3];Shou, Li-Bin[1,2,3];Lin, Dan-Dan[1,2,3];Zhou, Lei[1,2,3];Yang, Shi-Zhong[1,2,3];Liu, Jin-Feng[1,2,3];Li, Wei[4,5];Gu, Ji-Dong[6];Mu, Bo-Zhong[1,2,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Engn Res Ctr MEOR, Shanghai 200237, Peoples R China;[4]Shanghai Inst Pollut Control & Ecol Secur, Shanghai 200092, Peoples R China;[5]East China Univ Sci & Technol, Sch Resources & Environm Engn, Natl Engn Lab Ind Wastewater Treatment, Shanghai 200237, Peoples R China;[6]Guangdong Technion Israel Insu Tute Technol, Environm Engn, Shantou 515063, Guangdong, Peoples R China

年份：2020

卷号：54

期号：17

起止页码：10610

外文期刊名：ENVIRONMENTAL SCIENCE & TECHNOLOGY

收录：;EI(收录号：20204209347292);WOS:【SCI-EXPANDED(收录号:WOS:000568649900022)】；

基金：This work was supported by the National Natural Science Foundation of China (Grant No. 41807324, 41530318, 41373070, 51808223), Fundamental Research Funds for the Central Universities (Nos. 22221818014, 50321101917017, and JKJ012016028) and the Research Program of State Key Laboratory of Bioreactor Engineering to Y.F.L, S.M.M, W.L., and B.Z.M, the NSFC/RGC Joint Research Fund (No. 41161160560) to J.D.G. Correspondence and requests for materials should be addressed to B.Z.M.

语种：英文

外文关键词：Petroleum reservoirs - Petroleum reservoir engineering - Addition reactions - Carbon dioxide - Methane

摘要：Microbial anaerobic alkane degradation is a key process in subsurface oil reservoirs and anoxic environments contaminated with petroleum, with a major impact on global carbon cycling. However, the thermophiles capable of water-insoluble paraffins (>C-17) degradation under methanogenic conditions has remained understudied. Here, we established thermophilic (55 degrees C) n-paraffins-degrading (C-21-C-30) cultures from an oil reservoir. After over 900 days of incubation, the even-numbered n-paraffins were biodegraded to methane. The bacterial communities are dominated by a novel class- level lineage of actinobacteria, 'Candidatus Syntraliphaticia'. These 'Ca. Syntraliphaticia'-like metagenome-assembled genomes (MAGs) encode a complete alkylsuccinate synthases (ASS) gene operon, as well as hydrogenases and formate dehydrogenase, and several enzymes potentially involved in alkyl-CoA oxidation and the Wood-Ljungdahl pathway. Metatranscriptomic analysis suggests that n-paraffins are activated via fumarate addition reaction, and oxidized into carbon dioxide, hydrogen/formate and acetate by 'Ca. Syntraliphaticia', that could be further converted to methane by the abundant hydrogenotrophic and acetoclastic methanogens. We also found a divergent methyl-CoM reductase-like complex (MCR) and a canonical MCR in two MAGs representing 'Ca. Methanosuratus' (within candidate phylum Verstraetearchaeota), indicating the capability of methane and short-chain alkane metabolism in the oil reservoir. Ultimately, this result offers new insights into the degradability and the mechanisms of n-paraffins under methanogenic conditions at high temperatures.