文献类型：期刊文献

英文题名：[Effects of adenine on the growth and metabolism of Escherichia coli DH5alpha and its acetate-tolerant mutant DA19].

作者：Zhang, Xiao-yun[1];Zhang, Yan-jun;Li, Zhi-min;Xia, Yue-lan;Ye, Qin

机构：[1]State Key Laboratory of Bioreactor Engineering, East China University of Science & Technology, Shanghai 200237, China. zhangxiaoyungu@126.com

年份：2007

卷号：47

期号：3

外文期刊名：Wei sheng wu xue bao = Acta microbiologica Sinica

收录：MEDLINE(收录号：17672300);;PubMed

语种：英文

摘要：Batch cultures of Escherichia coli DH5alpha and DA19 in carbon source limited defined media and continuous cultures in nitrogen source limited defined media were performed to compare the metabolism in both strains. DH5alpha showed improved growth and less acetic acid production after supplementation of adenine, but adenine had little effects on the metabolism of DA19. The proteomic analysis showed that expression of purH was down regulated in DA19 by addition of adenine while was unchanged in DH5alpha, suggesting that the de novo synthesis ability of purine nucleotide in DH5a was poorer than that in DA19. Supplementation of adenine resulted in enhanced expression of gnd and yddS in DH5a, and thus improved the growth.