文献类型：期刊文献

英文题名：Total Synthesis and Immunological Evaluation of the Tri-D-glycero-D-manno-heptose Antigen of the Lipopolysaccharide as a Vaccine Candidate against Helicobacter pylori

作者：Wang, Junchang[1];Zhang, Yiyue[1];Zhu, Yirong[1];Liu, Junru[1];Chen, Yan[1];Cao, Xin[2];Yang, You[1]

机构：[1]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, Shanghai 200237, Peoples R China;[2]Fudan Univ, Inst Clin Sci, Shanghai Med Coll, Zhongshan Hosp, Shanghai 200032, Peoples R China

年份：2020

卷号：22

期号：22

起止页码：8780

外文期刊名：ORGANIC LETTERS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000592988800007)】；

基金：Financial support from the National Natural Science Foundation of China (21871081 and 22071054) and the Fundamental Research Funds for the Central Universities (50321031916002 and 22221818014) is gratefully acknowledged.

语种：英文

摘要：Helicobacter pylori, the most common cause of chronic gastritis, peptic ulcers, and gastric cancers, infects around half of the world's population. Although the drawbacks of antibiotic-based combination therapy are emerging, no effective vaccine is available to prevent H. pylori infections. Here, we describe the total synthesis of the unique alpha-(1 -> 3)-linked tri-D-glycero-D-mannoheptose antigen from the lipopolysaccharide of H. pylori serogroups O3 and O6 and strains MO19, D2, D4, and D5 based on de novo synthesis of the differentially protected D-glycero-D-manno-heptosyl building blocks. Immunization of mice with the semisynthetic glycoconjugate elicited a very robust T-cell-dependent antigen-specific immune response, resulting in very high titers of IgG1 and IgG2b protective antibody isotypes. The postimmune sera recognized H. pylori NCTC 11637 and bound strongly to the surface of the intact bacteria.