文献类型：期刊文献

英文题名：Improving the Oxygenation Performance of a Cyanobacterial Lipoxygenase by Oxygen Channel Engineering

作者：Qi, Yi-Ke[1];Zheng, Yu-Cong[1];Chen, Qi[1];He, Yucai[2];Zhang, Zhi-Jun[1];Xu, Jian-He[1]

机构：[1]East China Univ Sci & Technol, Shanghai Collaborat Innovat Ctr Biomfg, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Hubei Univ, Sch Life Sci, State Key Lab Biocatalysis & Enzyme Engn, Wuhan 430062, Peoples R China

年份：2021

卷号：9

期号：37

起止页码：12514

外文期刊名：ACS SUSTAINABLE CHEMISTRY & ENGINEERING

收录：;EI(收录号：20213810918262);WOS:【SCI-EXPANDED(收录号:WOS:000699846000006)】；

基金：This work was financially supported by the National Key Research and Development Program of China (No. 2019YFA0905000), National Natural Science Foundation of China (Nos. 21536004, 21776085, 21871085, & 32071475), Natural Science Foundation of Shanghai (No. 18ZR1408400), Fundamental Research Funds for the Central Universities (No. 22221818014) and Open Funding Project of the State Key Laboratory of Biocatalysis and Enzyme Engineering (Hubei University). The authors thank the funders for the support.

语种：英文

外文关键词：Biocatalysis; Lipoxygenase; Oxygen channel; Enzyme engineering Hydroxy fatty acids

摘要：Lipoxygenase catalyzes the dioxygenation of unsaturated fatty acids, which represents a sustainable pathway for producing bioderived fine chemicals. Herein, structure-guided engineering of the oxygen tunnel of a well-expressed 13-(S)-lipoxygenase from cyanobacteria was performed, resulting in a mutant A324G/S392G which showed 3 to 5-fold increases in activity toward a panel of unsaturated fatty acids. Molecular dynamics simulations elucidated the molecular basis for A324 and S392 as activity determining residues in the oxygen tunnel. The practicability of the mutant was demonstrated by the biosynthesis of hydroperoxy fatty acid 13S-HPOD with a high space time yield (STY) of 2.28 kg L-1 d(-1). Our results also demonstrate a good example for engineering biocatalysts that require gaseous molecules as cosubstrate.