文献类型：期刊文献

英文题名：Impacts of proline on the central metabolism of an industrial erythromycin-producing strain Saccharopolyspora erythraea via 13C labeling experiments

作者：Hong, Ming[1];Huang, Mingzhi[1];Chu, Ju[1];Zhuang, Yingping[1];Zhang, Siliang[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2016

卷号：231

起止页码：1

外文期刊名：JOURNAL OF BIOTECHNOLOGY

收录：;EI(收录号：20162702564429);WOS:【SCI-EXPANDED(收录号:WOS:000380242800001)】；

基金：This work was financially supported by the National Basic Research Program of China (973 Program) (No. 2012CB721006), National Natural Science Foundation of China (No. 21276081), the National Scientific and Technological Major Special Project (Significant Creation of New drugs), No. 2011ZX09203-001-03, Research Fund for the Doctoral Program of Higher Education of China (No. 20110074110015).

语种：英文

外文关键词：C-13 metabolic flux analysis; Proline; Saccharopolyspora erythraea; Chemically defined medium

摘要：Saccharopolyspora erythraea E3 is an important industrial strain for erythromycin production and knowledge on its metabolism is limited. In the present work, C-13 labeling experiments were conducted to characterize the metabolism of S. erythraea E3. We found that S. erythraea E3 was difficult to grow on minimal medium with glucose as sole carbon source and the addition of proline remarkably improved the cell growth. The activity of EMP pathway was very low and ED pathway was alternatively the main glucose utilization pathway. The addition of proline resulted in remarkable changes in the fluxes of central metabolism. The fluxes in PP pathway, in TCA cycle and in ED pathway were 90% higher, 64% and 31% lower on Glc/Pro than on Glc, respectively. The maintenance energy on Glc/Pro was 58.4% lower than that on Glc. The energy charge was lower on Glc than on Glc/Pro, indicating that the cells on Glc suffered from energy burden. This study elucidates the impacts of proline on the central metabolism of S. erythraea and deepens the understanding of its metabolism. (C) 2016 Elsevier B.V. All rights reserved.