文献类型：期刊文献

英文题名：Detection of Toxoplasma gondii oocysts in water sample concentrates by real-time PCR

作者：Yang, Wenli[1,2]; Lindquist, H. D. Alan[3]; Cama, Vitaliano[1]; Schaefer III, Frank W.[3]; Villegas, Eric[4]; Fayer, Ronald[5]; Lewis, Earl J.[6]; Feng, Yaoyu[7]; Xiao, Lihua[1,8]

机构：[1] Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30341, United States; [2] Atlanta Research and Education Foundation, 1670 Clairmont Road, Decatur, GA 30033, United States; [3] National Homeland Security Research Center, United States Environmental Protection Agency, Cincinnati, OH 45268, United States; [4] National Exposure Research Laboratory, United States Environmental Protection Agency, Cincinnati, OH 45268, United States; [5] Agricultural Research Service, United States Department of Agriculture, Beltsville, MD 20705, United States; [6] Center for Coastal Environmental Health and Biomolecular Research, National Oceanic and Atmospheric Administration, Oxford, MD 21654, United States; [7] School of Resource and Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China; [8] Division of Parasitic Diseases, National Center for Zoonotic, Vector-Borne and Enteric Diseases, Centers for Disease Control and Prevention, 4770 Buford Highway, Atlanta, GA 30341, United States

年份：2009

卷号：75

期号：11

起止页码：3477

外文期刊名：Applied and Environmental Microbiology

收录：EI(收录号：20092412119865)

语种：英文

外文关键词：Assays - Polymerase chain reaction - Surface waters

摘要：PCR techniques in combination with conventional parasite concentration procedures have potential for the sensitive and specific detection of Toxoplasma gondii oocysts in water. Three real-time PCR assays based on the B1 gene and a 529-bp repetitive element were analyzed for the detection of T. gondii tachyzoites and oocysts. Lower sensitivity and specificity were obtained with the B1 gene-based PCR than with the 529-bp repeat-based PCR. New procedures for the real-time PCR detection of T. gondii oocysts in concentrates of surface water were developed and tested in conjunction with a method for the direct extraction of inhibitor-free DNA from water. This technique detected as few as one oocyst seeded to 0.5 ml of packed pellets from water samples concentrated by Envirocheck filters. Thus, this real-time PCR may provide a detection method alternative to the traditional mouse assay and microscopy. Copyright ? 2009, American Society for Microbiology. All Rights Reserved.