文献类型：期刊文献

中文题名：蛋白组学差异揭示黑曲霉(Aspergillus niger)高产柠檬酸的分子机制

英文题名：Molecular mechanism revealed by proteomic differences of Aspergillus niger with high yield citric acid

作者：谢慧[1,2];王风清[1];魏东芝[1]

机构：[1]华东理工大学生物反应器国家重点实验室,上海200237;[2]河南农业大学生命科学学院,河南郑州450002

年份：2018

卷号：37

期号：7

起止页码：138

中文期刊名：中国酿造

外文期刊名：China Brewing

收录：CSTPCD;;北大核心:【北大核心2017】；

基金：国家重大科技计划项目(No.2012ZX09304009)

语种：中文

中文关键词：二维聚丙烯酰胺凝胶电泳;同位素标记相对和绝对定量;蛋白质组学技术;柠檬酸;黑曲霉

外文关键词：2-DE;iTRAQ;proteomics technology;citric acid;Aspergillus niger

摘要：黑曲霉(Aspergillus niger)YX-1217是一株典型的柠檬酸高产菌株,基于二维聚丙烯酰胺凝胶电泳(2-DE)对黑曲霉YX-1217和退化菌株YX-1217G的胞外分泌蛋白进行比较,同时利用同位素标记相对和绝对定量(i TRAQ)技术比较了两株菌的胞内蛋白差异,揭示黑曲霉YX-1217高产柠檬酸的分子机制。结果显示,菌株黑曲霉YX-1217G的胞外蛋白数量和浓度均显著低于菌株黑曲霉YX-1217(P<0.05),通过质谱鉴定了6个黑曲霉YX-1217高表达蛋白;利用i TRAQ技术共鉴定到3 553个蛋白,将差异蛋白按照京都基因与基因组百科全书(KEGG)代谢数据库进行分类,发现与柠檬酸生物合成相关的酶在菌株黑曲霉YX-1217中的表达量显著高于退化菌株(P<0.05),这些蛋白的高效表达是菌株黑曲霉YX-1217高产柠檬酸的原因之一,为开发柠檬酸或其他有机酸的高产菌株提供新的思路和观点。
Aspergillus niger YX-1217 is a typical high citric-acid-producing strain. Based on the two proteomics techniques of two-dimensional polyacrylamide gel electrophoresis （2-DE） and isobaric tags for relative and absolute quantification （iTRAQ）, the exocytosis proteins and intracellular proteins of A. niger YX-1217 and degenerative A. niger YX-1217G were compared and the differential proteins involved in citric acid production were analyzed, which revealed the mechanism of A. niger YX-1217 with citric acid production. The results showed that the amount and concentration of extracellular proteins of A. niger YX-1217G were significantly lower than that of A. niger YX-1217 （P〈0.05）, and six overexpression proteins were identified by mass spectrometry. A total of 3 553 intracellular proteins were identified by iTRAQ technique. The differential proteins were classified according to the Kyoto Encyclopedia of Genes and Genomes （KEGG） database and the results showed that the expression level of proteins involved in citric acid biosynthesis in strain A. niger YX-1217 was significantly higher than that in degenerative strain A. niger YX-1217G （P〈0.05）. The high expression of these proteins was obviously one of the important reasons for the citric acid production of strain A. niger YX-1217, which would provide new ideas for the development of strains with high yield citric acid or other organic acids.