文献类型：期刊文献

英文题名：Surface Morphology and the Action Mechanism of Macrophage Immuno-Stimulating Activity of Rosa Setate X Rosa Rugosa Polysaccharide Wsrp-1b

作者：Wu, Mengqi[1,2]; Xu, Jingying[2]; Li, Wei[3]; Han, Qifeng[2]; Xia, Wei[2]; Li, Zhong[1]; Zhang, Yang[1]; Zhang, Wenqing[2]

机构：[1] Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai, 200237, China; [2] Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, China; [3] State Key Laboratory of Dairy Biotechnology, Shanghai Engineering Research Center of Dairy Biotechnology, Dairy Research Institute, Bright Dairy & Food Co., Ltd., Shanghai, 200436, China

年份：2023

外文期刊名：SSRN

收录：EI(收录号：20230140368)

语种：英文

外文关键词：Antigen-antibody reactions - Azo dyes - Disease control - Electrophoresis - Morphology - Nitric oxide - Scanning electron microscopy - Surface morphology

摘要：Our previous research demonstrated that a glucomannan WSRP-1b separated from waste biomass of Rosa Setate x Rosa Rugosa had potent immunostimulatory activity. However, the specific mechanisms involved in the activation of RAW264.7 macrophages by WSRP-1b are still unclear. Scanning electron microscopy, atomic force microscopy and Congo red analyses uncovered that WSRP-1b exhibited a flake structure with irregular curls, and had a triple-helix conformation in solution. The immunostimulating assay showed that WSRP-1b could notably enhance phagocytosis of macrophages, and promote the accumulation of iNOS, TNF-α and IL-6 mRNA. Western blot analysis indicated the expression of the phosphorylation of mitogen-activated protein kinases (MAPKs) was upregulated in WSRP-1b-induced RAW264.7 cells. Furthermore, pretreatment of RAW 264.7 cells using specific inhibitors against the MAPKs and NF-κB (nuclear factor-κB) remarkably attenuated NO (nitric oxide) production and cytokines secretion. These data suggested that WSRP-1b activated RAW264.7 macrophages via MAPK and NF-κB signaling pathways and might be a novel immunomodulatory agent for hypoimmunity diseases therapy. ? 2023, The Authors. All rights reserved.