文献类型：期刊文献

英文题名：Design of a palette of SNAP-tag mimics of fluorescent proteins and their use as cell reporters

作者：Zhang, Dasheng[1,2];Chen, Zhengda[1,2];Du, Zengmin[1,2];Bao, Bingkun[1,3];Su, Ni[1,2];Chen, Xianjun[1,2];Ge, Yihui[1,2];Lin, Qiuning[1,3];Yang, Lipeng[1,3];Hua, Yujie[1,3];Wang, Shuo[1,2];Hua, Xin[1,3];Zuo, Fangting[1,2];Li, Ningfeng[1,3];Liu, Renmei[1,2];Jiang, Li[1,3];Bao, Chunyan[1];Zhao, Yuzheng[1,2];Loscalzo, Joseph[4];Yang, Yi[1,2];Zhu, Linyong[1,2,3]

机构：[1]East China Univ Sci & Technol, Optogenet & Synthet Biol Interdisciplinary Res Ctr, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[2]East China Univ Sci & Technol, Shanghai Frontiers Sci Ctr Optogenet Tech Cell Met, Sch Pharm, Shanghai, Peoples R China;[3]Shanghai Jiao Tong Univ, Sch Biomed Engn, Shanghai, Peoples R China;[4]Harvard Med Sch, Brigham & Womens Hosp, Dept Med, Boston, MA USA

年份：2023

卷号：9

期号：1

外文期刊名：CELL DISCOVERY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001006147300001)】；

基金：AcknowledgementsWe thank Ting Li, Rongkun Tao, Hanyang Hu, and Wentong Zhu for technical assistance, and Stephanie Tribuna for secretarial assistance. We thank Prof. Jiahuai Han at Xiamen University for providing CX43 cDNA. We thank Dr. Lili Lv and Dr. Qijun Zhang at Shanghai Medicilon Inc. for discussion of atomic structure of the SmFP. We thank Songzi Jiang and Zhijun Liu at Chinese Academy of Sciences for technical support. This research was supported by the National Key Research and Development Program of China (2022YFC3400100 to Y.Y. and X.C., 2019YFA0110500 to L.Z., 2019YFA0904800 to Y.Y. and L.Z.), NSFC (32121005, 32150028, 21937004 and 91857202 to Y.Y.; 21907029 and 21877037 to L.Z.; 32250009 to X.C.), STI2030-Major Projects (2021ZD0202200 and 2021ZD0202203 to X.C.), the Shanghai Municipal Education Commission (2021 Sci & Tech 03-28) to L.Z., Y.Y. and X.C., the Shanghai Rising-Star Program to X.C., the State Key Laboratory of Bioreactor Engineering to Y.Y. and X.C., the Fundamental Research Funds for the Central Universities to Y.Y. and X.C.. J.L. was supported by US NIH grants HL61795, HG007690, and GM 107618.

语种：英文

摘要：Naturally occurring fluorescent proteins (FPs) are the most widely used tools for tracking cellular proteins and sensing cellular events. Here, we chemically evolved the self-labeling SNAP-tag into a palette of SNAP-tag mimics of fluorescent proteins (SmFPs) that possess bright, rapidly inducible fluorescence ranging from cyan to infrared. SmFPs are integral chemical-genetic entities based on the same fluorogenic principle as FPs, i.e., induction of fluorescence of non-emitting molecular rotors by conformational locking. We demonstrate the usefulness of these SmFPs in real-time tracking of protein expression, degradation, binding interactions, trafficking, and assembly, and show that these optimally designed SmFPs outperform FPs like GFP in many important ways. We further show that the fluorescence of circularly permuted SmFPs is sensitive to the conformational changes of their fusion partners, and that these fusion partners can be used for the development of single SmFP-based genetically encoded calcium sensors for live cell imaging.