文献类型：期刊文献

英文题名：A new NAD(H)-dependent meso-2,3-butanediol dehydrogenase from an industrially potential strain Serratia marcescens H30

作者：Zhang, Liaoyuan[1];Xu, Quanming[1];Zhan, Senran[1];Li, Yongyu[3];Lin, Hui[1];Sun, Shujing[1];Sha, Li[1];Hu, Kaihui[1];Guan, Xiong[1];Shen, Yaling[2]

机构：[1]Fujian Agr & Forestry Univ, Coll Life Sci, Minist Educ, Key Lab Biopesticide & Chem Biol, Fuzhou 350002, Fujian Province, Peoples R China;[2]E China Univ Sci & Technol, New World Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[3]Fujian Agr & Forestry Univ, Coll Hort, Fuzhou 350002, Fujian Province, Peoples R China

年份：2014

卷号：98

期号：3

起止页码：1175

外文期刊名：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

收录：;EI(收录号：20141717605297);WOS:【SCI-EXPANDED(收录号:WOS:000333612000019)】；

基金：This work was supported by the Open Funding Project of the State Key Laboratory of Bioreactor Engineering, the Natural Science Foundation of Fujian Province of China (no. 2011J05048) and Science Fund of the Provincial Education Department of Fujian Province of China (no. JA11089).

语种：英文

外文关键词：Serratia marcescens; Meso-2,3-butanediol dehydrogenase; Cloning; Expression; Enzymatic properties

摘要：The budC gene coding for a new meso-2,3-butanediol dehydrogenase (BDH) from Serratia marcescens H30 was cloned and expressed in Escherichia coli BL21(DE3), purified, and characterized for its properties. The recombinant BDH with a molecular weight of 27.4 kDa exhibited a reversible transformation between acetoin and 2,3-butanediol. In the presence of NADH, BDH could catalyze the reduction of diacetyl and (3R)-acetoin to (3S)-acetoin and meso-2,3-butanediol, respectively, while (3S)-acetoin as a substrate could be further transformed into (2S, 3S)-2,3-butanediol at pH 9.0. For diol oxidation reactions, (3R)-acetoin and (3S)-acetoin were obtained when meso-2,3-butanediol and (2S,3S)-2,3-butanediol were used as the substrates with BDH and NAD(+). (2R,3R)-2,3-butanediol was not a substrate for the BDH at all. The low K (m) value (4.1 mM) in meso-2,3-butanediol oxidation reaction and no activity for diacetyl, acetoin, and 2,3-butanediol as the substrates with NADP(+)/NADPH suggested that the budC gene product belongs to a NAD(H)-dependent meso-2,3-BDH. Maximum activities for diacetyl and (3S/3R)-acetoin reduction were observed at pH 8.0 and pH 5.0 while for meso-2,3-butanediol oxidation it was pH 8.0. However, the optimum temperature for oxidation and reduction reactions was about 40 A degrees C. In addition, the BDH activity for meso-2,3-butanediol oxidation was enhanced in the presence of Fe2+ and for diacetyl and (3S/3R)-acetoin reduction in the presence of Mg2+ and Mn2+, while several metal ions inhibited its activity, particularly Fe3+ for reduction of diacetyl and acetoin. Sequence analysis showed that the BDH from S. marcescens H30 possessed two conserved sequences including the coenzyme binding motif (GxxxGxG) and the active-site motif (YxxxK), which are present in the short-chain dehydrogenase/reductase superfamily.