文献类型：期刊文献

英文题名：Synthetic yeast-bacterium consortium enables co-inducible relayed synthesis of chemicals

作者：Xu, Mingqiang[1];Yu, Jiahui[1];Chen, Xinjie[1];Li, Xie[1,2,3];Guo, Yan[1];Qian, Zhilan[1];Liu, Qi[1];Ren, Yanna[1];Lu, Jian[1];Chen, Xianjun[1,2,3,4];Yang, Yi[1,2,3,4];Cai, Menghao[1,2,4]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[2]East China Univ Sci & Technol, Shanghai Frontiers Sci Ctr Optogenet Tech Cell Met, Sch Pharm, Shanghai, Peoples R China;[3]East China Univ Sci & Technol, Optogenet & Synthet Biol Interdisciplinary Res Ctr, Shanghai, Peoples R China;[4]East China Univ Sci & Technol, Shanghai Collaborat Innovat Ctr Biomfg, Shanghai, Peoples R China

年份：2026

卷号：97

外文期刊名：METABOLIC ENGINEERING

收录：;EI(收录号：20263021165041);Scopus(收录号：2-s2.0-105045437797);WOS:【SCI-EXPANDED(收录号:WOS:001834911000001)】；

基金：This work was supported by the National Key Research and Development Program of China (2023YFA0914100/02) ; the National Key Research and Development Program of China (2022YFC3400100) ; the National Key Research and Development Program of China (2020YFA0907800) ; the Shanghai Explorer Program (24TS1411600) ; and the National Natural Science Foundation of China (32121005)

语种：英文

外文关键词：Synthetic consortiums; Co-inducible; Quorum sensing; Optogenetics; Komagataella phaffii; Escherichia coli

摘要：Microbial coculture can integrate advantages and overcome the metabolic imbalance of individual species. Programming strain interactions represents a common routine for synthetic microbial communities with distinct species, which causes difficulties and redundant workloads in interaction construction before being available as chassis hosts. This study explores yeast-bacterium consortium without engineered interactions for the coinducible relayed synthesis of natural products. The Komagataella phaffii-Escherichia coli consortium is explored for co-growth under selected conditions. Low-level glucose- and blue light-responsive transcriptional systems are rebuilt separately for each host, allowing single-signal co-induced activation of compound synthesis in coculture. Pathway redirection, genome mining, and rewiring of key targets for acyl donor degradation result in efficient production of the reporter molecule simvastatin (26.2 mg l- 1) through living consortium cultured on simple carbon source. Inducible biosynthesis of another reporter compound (2S)-naringenin (165.6 mg l- 1) further validates the extendibility of this community. The described platform represents a breakthrough in engineering microbial consortium for biosynthesis.