文献类型：期刊文献

英文题名：A signal-amplifiable biochip quantifies extracellular vesicle-associated RNAs for early cancer detection

作者：Hu, Jiaming[1];Sheng, Yan[1,2];Kwak, Kwang Joo[1];Shi, Junfeng[1];Yu, Bohao[3];Lee, L. James[1]

机构：[1]Ohio State Univ, Dept Chem & Biomol Engn, Columbus, OH 43210 USA;[2]Yantai Univ, Coll Chem & Chem Engn, Yantai, Peoples R China;[3]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China

年份：2017

卷号：8

外文期刊名：NATURE COMMUNICATIONS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000416226200012)】；

基金：This research was funded by The Ohio State University Development Funds. Y.S. appreciated National Natural Science Foundation of China (No. 31300789) and Natural Science Foundation of Shandong Province (No. ZR2013CQ016) for partial financial support. The human serum samples were kindly donated from the First Affiliated Hospital of Bengbu Medical College and Jiangsu Xuzhou Third People's Hospital, China.

语种：英文

摘要：Detection of extracellular vesicle (EV)-associated RNAs with low expression levels in early-stage cancer remains a challenge and is highly valuable. Here, we report a nanoparticle-based biochip that could capture circulating EVs without isolation, brighten encapsulated RNAs, and amplify fluorescence signals in situ in a single step. We confine catalyzed hairpin DNA circuit (CHDC) in cationic lipid-polymer hybrid nanoparticles (LPHNs) that are tethered on a chip. LPHN features a core-shell-corona structure that facilitates the transfer and mixing of CHDC with EV-associated RNAs when forming the LPHN-EV nanocomplex. CHDC is triggered upon target RNA binding and quickly generate amplified signals. The signal amplification efficiency of LPHN-CHDC is demonstrated in artificial EVs, cancer cells, and cancer cell-derived EVs. We show that LPHN-CHDC biochip with signal amplification capability could selectively and sensitively identify low expression glypican-1 mRNA in serum EVs, distinguishing patients with early-and late-stage pancreatic cancer from healthy donors and patients with benign pancreatic disease.