文献类型：期刊文献

英文题名：One-pot synthesis of glutathione by a two-enzyme cascade using a thermophilic ATP regeneration system

作者：Zhang, Xing[1];Wu, Hui[1];Huang, Bing[1];Li, Zhimin[1,2];Ye, Qin[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Shanghai Collaborat Innovat Ctr Biomfg Technol, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2017

卷号：241

起止页码：163

外文期刊名：JOURNAL OF BIOTECHNOLOGY

收录：;EI(收录号：20165003125982);WOS:【SCI-EXPANDED(收录号:WOS:000392677900022)】；

基金：This study was supported by the National Science Foundation for Young Scientist of China (Grant No. 21406065), the Fundamental Research Funds for the Central Universities (Grant No. 222201313007 & 22A201514042), and Shanghai Committee of Science and Technology (Grant No. 13DZ1930202). Partially supported by Open Funding Project of the State Key Laboratory of Bioreactor Engineering. Partially supported by the Foundation of Key Laboratory for Industrial Biocatalysis (Tsinghua University), Ministry of Education (Grant No. 2015101).

语种：英文

外文关键词：Glutathione; Bifunctional glutathione synthetase; ATP regeneration; Polyphosphate; Polyphosphate kinase

摘要：In vitro cascade catalysis using enzyme -based system is becoming a promising biomanufacturing platform for biofuels and biochemicals production. Glutathione is a pivotal non-protein thiol compound and widely applied in food and pharmaceutical industries. In this study, glutathione was synthesized by a bifunctional glutathione synthetase together with a thermophilic ATP regeneration system through a two-enzyme cascade in vitro. Four bifunctional glutathione synthetases from Streptococcus sanguinis, S. gordonii, S. uberis and Bacillus cereus were applied for glutathione synthesis. The bifunctional glutathione synthetase from S. sanguinis was selected and coupled with the polyphosphate kinase from Thermosynechococcus elongatus BP-1 for regenerating ATP to produce glutathione id one pot. In the optimized system, 28.5 mM glutathione was produced within 5 h due to efficient ATP regeneration from low-cost polyphosphate. The yield based on added L-cysteine reached 81.4% and the productivity of glutathione achieved 5.7 mM/h. The one-pot system indicated a potential biotransformation platform for industrial production of glutathione. (C) 2016 Elsevier B.V. All rights reserved.