文献类型：期刊文献

英文题名：Efficient isopropanol biosynthesis by engineered Escherichia coli using biologically produced acetate from syngas fermentation

作者：Yang, Hao[1];Zhang, Can[2];Lai, Ningyu[1];Huang, Bing[1];Fei, Peng[1];Ding, Dawei[3];Hu, Peng[3];Gu, Yang[2];Wu, Hui[1,4,5]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Key Lab Synthet Biol, Shanghai 200032, Peoples R China;[3]Shanghai GTL Biotech Co Ltd, 1688 North Guoquan Rd, Shanghai 200438, Peoples R China;[4]China Natl Light Ind Council, Key Lab Biobased Mat Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[5]Shanghai Collaborat Innovat Ctr Biomfg Technol, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2020

卷号：296

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20194807736262);WOS:【SCI-EXPANDED(收录号:WOS:000500462400021)】；

基金：This study was supported by the Fok Ying-Tong Education Foundation, China (Grant No. 161017), the National Key Research and Development Program of China (Grant No. 2017YFB0309302), the National Natural Science Foundation of China (Grant No. 21776083), the Natural Science Foundation of Shanghai, the Fundamental Research Funds for the Central Universities (Grant No. 22221818014), the 111 Project (B18022), the Science and Technology Commission of Shanghai Municipality (Grant No. 17JC1404800). Partially supported by Open Funding Project of the State Key Laboratory of Bioreactor Engineering.

语种：英文

外文关键词：Acetate; Isopropanol; Metabolic engineering; Cofactor engineering; Escherichia coli

摘要：The shortage of food based feedstocks is a challenge in industrial biomanufacturing. In this study, genetically modified Escherichia coli strains were used to produce isopropanol as the mainly product from acetate, a cost-effective nonfood-based substrate. The isopropanol biosynthesis pathway was constructed by combining genes from Clostridium acetobutylicum (thlA, adc), E. coli (atoDA) and Clostridium beijerinckii (adh). E. coli MG1655 harboring the isopropanol biosynthesis pathway successfully produced isopropanol and low amounts of acetone from pure acetate. The enhancement of the acetate assimilation pathway coupled with cofactor engineering strategy further improved the production of isopropanol to 18.5mM with a yield of 0.26 mol/mol. With simple treatment, two kinds of biologically produced acetate were utilized to generate 16.7 and 24.5mM isopropanol with yields of 0.25 and 0.56 mol/mol, respectively. Engineered E. coli with an optimized isopropanol biosynthesis pathway can efficiently utilize biologically produced acetate to synthesize isopropanol.