文献类型：期刊文献

英文题名："Crossbreeding" Small-Molecular Weight NIR-II Flavchromenes Endows Activatable Multiplexed In Vivo Imaging

作者：Zhang, Liao[1,2];Zhang, Yutao[1,2];Chi, Weijie[3];Yan, Chenxu[1,2];Zhao, Zijun[1,2];Liu, Xiaogang[3];Zhu, Wei-Hong[1,2];Guo, Zhiqian[1,2]

机构：[1]East China Univ Sci & Technol, Inst Fine Chem, Sch Chem & Mol Engn, Key Lab Adv Mat,Feringa Nobel Prize Scientist Joi, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Inst Fine Chem, Sch Chem & Mol Engn, Joint Int Res Lab Precis Chem & Mol Engn,Feringa, Shanghai 200237, Peoples R China;[3]Singapore Univ Technol & Design, Fluorescence Res Grp, Singapore 487372, Singapore

年份：2022

卷号：4

期号：8

起止页码：1493

外文期刊名：ACS MATERIALS LETTERS

收录：;EI(收录号：20223412614110);WOS:【SCI-EXPANDED(收录号:WOS:000863556100001)】；

基金：This work was supported by NSFC/China (21878087 and 21908060), National Key Research and Development Program (2021YFA0910000), Shanghai Municipal Science and Technology Major Project (Grant 2018SHZDZX03), Shanghai Frontier Science Research Base of Optogenetic Techniques for Cell Metabolism (Shanghai Municipal Education Commission, grant 2021 Sci & Tech 03-28), A*STAR under its Advanced Manufacturing and Engineering Program (A2083c0051), the Ministry of Education, Singapore (MOE-MOET2EP101200007), and the Singapore University of Technology and Design [SUTD-ZJU (VP)201905]. The authors to acknowledge the use of the computing service of SUTD-MIT IDC and the National Supercomputing Centre, Singapore.

语种：英文

外文关键词：Biocompatibility - Bioimaging - Chemical stability - Fluorophores - Infrared devices

摘要：High-performance near-infrared-II (NIR-II) fluorophores have attracted tremendous attention for in vivo dynamic bioimaging. However, the lack of stable, bright, and biocompatible molecular fluorophores becomes a major barrier on the way to the widespread endorsement of NIR-II bioimaging for clinic diagnosis. Here we propose a pi-conjugated "crossbreeding" dyad strategy to develop novel desirable NIR-II dyes, flavchromenes (flav+chromene) integrated from two individual fluorophores of flavylium and chromene via a short methine bridge, affording valuable features of extending spectra into the NIR-II region and conferring a substantive leap in the photophysical properties. Notably, these crossbreeding NIR-II dyes have small molecular weights, high molar extinction coefficients, and outstanding chemical stability. As demonstrated, the elaborated probe Flavchrom-4 endows activatable dual-modal in vivo imaging of endogenous ss-galactosidase activity, along with lighting-up NIR-II fluorescence signals and dramatically enhanced photoacoustic signals (16.3-fold). This concise pi-conjugated crossbreeding dyad strategy brings forth high-performance NIR-II fluorophore scaffolds with small molecular weights, high stability, and good brightness, thus greatly expanding high-resolution activatable in vivo imaging tools for intact tissues and living animals.