文献类型：期刊文献

英文题名：A New Prodrug-Derived Ratiometric Fluorescent Probe for Hypoxia: High Selectivity of Nitroreductase and Imaging in Tumor Cell

作者：Cui, Lei[1];Zhong, Ye[1];Zhu, Weiping[1];Xu, Yufang[1];Du, Qingshan[1];Wang, Xin[1];Qian, Xuhong[1];Xiao, Yi[2]

机构：[1]E China Univ Sci & Technol, Sch Pharm, State Key Lab Bioreactor Engn, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China;[2]Dalian Univ Technol DLUT, Dalian, Peoples R China

年份：2011

卷号：13

期号：5

起止页码：928

外文期刊名：ORGANIC LETTERS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000287645800027)】；

基金：This work was financially supported by the Key New Drug Creation and Manufacturing Program (2009ZX09103-102), the National High Technology Research and Development Program of China (863 Program 2011AA10A207), the National Basic Research Program of China (973 Program, 2010CB126100), the China 111 Project (Grant B07023), and the Shanghai Leading Academic Discipline Project (13507). We appreciate Professor Eric V. Anslyn (Department of Chemistry & Biochemistry, The University of Texas at Austin) for the directions for the proposed detection mechanism. We also appreciate Professor Youjun Yang (School of Pharmacy, East China University of Science and Technology) for the improvements in this paper.

语种：英文

摘要：Based on the hypoxia prodrug moiety of p-nitrobenzyl, a selective ratiometric fluorescent sensor (RHP) for the detection of microenvironment hypoxia was designed and synthesized. RHP can be selectively activated by bioreductive enzymes (NTR) and results in an evident blue to green fluorescent emission wavelength change in both solution phases and in cell lines, which might be the first fluorescent ratiometric probe for hypoxia in solid tumors.