文献类型：期刊文献

英文题名：Physiological metabolic topology analysis of Halomonas elongata DSM 2581T in response to sodium chloride stress

作者：Yu, Junxiong[1];Wang, Zejian[1];Wang, Jing[2];Mohisn, Ali[1];Liu, Hao[1];Zhang, Yue[1];Zhuang, Yingping[1];Guo, Meijin[1]

机构：[1]East China Univ Sci & Technol, Dept Biotechnol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Dept Chem Engn Energy Resources, Shanghai, Peoples R China

年份：2022

卷号：119

期号：12

起止页码：3509

外文期刊名：BIOTECHNOLOGY AND BIOENGINEERING

收录：;EI(收录号：20223812774815);WOS:【SCI-EXPANDED(收录号:WOS:000855101900001)】；

基金：National Key R&D Program of China, Grant/Award Number: 2020YFA0906800; National Natural Science Foundation of China, Grant/Award Number: 22078295; National Natural Science Foundation of China, Grant/Award Number: 32071471; Postgraduate Research & Practice Innovation Program of Jiangsu Province, Grant/Award Number: KYCX21_2866; Fundamental Research Funds for the Central Universities, Grant/Award Number: 222201714024; Innovation and Intelligence 111 Plan; National Key R&D Program of China, Grant/Award Number: 2021YFC2101000

语种：英文

外文关键词：ectoine; Halomonas elongata DSM 2581(T); kinetic; NaCl stress; transcriptomic

摘要：The halophilic bacterium Halomonas elongata DSM 2581(T) generally adapts well to high level of salinity by biosynthesizing ectoine, which functions as an important compatible solute protecting the cell against external salinity environment. Halophilic bacteria have specific metabolic activities under high-salt conditions and are gradually applied in various industries. The present study focuses on investigating the physiological and metabolic mechanism of H. elongata DSM 2581(T) driven by the external salinity environment. The physiological metabolic dynamics under salt stress were investigated to evaluate the effect of NaCl stress on the metabolism of H. elongata. The obtained results demonstrated that ectoine biosynthesis transited from a nongrowth-related process to a growth-related process when the NaCl concentration varied from 1% to 13% (w/v). The maximum biomass (X-m = 41.37 g/L), and highest ectoine production (P-m = 12.91 g/L) were achieved under 8% NaCl. Moreover, the maximum biomass (X-m) and the maximum specific growth rates (mu(m)) showed a first rising and then declining trend with the increased NaCl stress. Furthermore, the transcriptome analysis of H. elongata under different NaCl concentrations demonstrated that both 8% and 13% NaCl conditions resulted in increased expressions of genes involved in the pentose phosphate pathway, Entner-Doudoroff pathway, flagellar assembly pathway, and ectoine metabolism, but negatively affected the tricarboxylic acid cycle and fatty acid metabolism. At last, the proposed possible adaptation mechanism under the optimum NaCl concentration in H. elongata was described.