文献类型：期刊文献

英文题名：Three-dimensional graphene-like homogeneous carbon architecture loaded with gold-platinum for the electrochemical detection of circulating tumor DNA

作者：Chen, K. C.[1];Zhao, H. L.[1];Wang, Z. X.[1];Lan, M. B.[1,2]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Res Ctr Anal & Test, Shanghai 200237, Peoples R China

年份：2022

卷号：24

外文期刊名：MATERIALS TODAY CHEMISTRY

收录：;EI(收录号：20221511940473);WOS:【SCI-EXPANDED(收录号:WOS:000793286200008)】；

基金：Acknowledgments This work was supported by the Science and Technology Commis-sion of Shanghai Municipality (STCSM, 20520712500, 20392002300) ; and the Fundamental Research Funds for the Central Universities (50321102117022) . The authors thank the Research Center of Analysis and Test of East China University of Science and Technology for the help of X-ray photoelectron spectrometer analysis.

语种：英文

外文关键词：Tumor biomarker; Three-dimensional architecture; Graphene-like carbon; Gold-platinum alloy; Electrochemical biosensor

摘要：The analysis of circulating tumor DNA (ctDNA) turns out to be increasingly significant considering its potential value in the clinical diagnosis of cancer. Herein, a ctDNA electrochemical biosensor was developed with a low detection limit and high selectivity by using three-dimensional graphene-like homogeneous carbon architecture (3D-GHC600) loaded with gold-platinum (AuPt). The 3D-GHC600 was derived from the annealing process of copper-based metal-organic framework (denoted as Cu-BTC) at the optimal temperature (600 ?), which includes the merits of large area, rich mesopores, homogeneous size and morphology, and 3D structure. AuPt was formed on the 3D-GHC(600) surface via an in-situ reduction reaction. Characterizations demonstrated that the resultant composite catalyst (AuPt/3DGHC600) was prepared successfully and exhibited remarkable electrochemical properties. Further, the catalyst was used as a label of signal probes (SPs) to form SPs-label. The hybridization reactions were completed by layer-by-layer recognition of capture probes (CPs), target DNA (tDNA), and SPs-label on the electrode, thus forming a sandwich-like structure. The current signals of the SPs-label toward the electrocatalytic reduction of H2O2 were recorded in all tests for tDNA analysis. Accordingly, this recommended biosensor of tDNA showed good performance including a wide linear range of 10(-8) M 10(-17) M with a detection limit of 2.25 x 10-18 M (S/N = 3), excellent selectivity for the recognition of different interferences, satisfying reproducibility, good stability, and outstanding recovery. These results demonstrated the promising application of the biosensor in the detection of ctDNA. (C)& nbsp;2022 Elsevier Ltd. All rights reserved.