文献类型：期刊文献

英文题名：Manipulation of VEGF-induced angiogenesis by 2-N, 6-O-sulfated chitosan

作者：Yu, Yuanman[1,2,3];Chen, Rui[1,2];Sun, Yi[1];Pan, Yuanzhong[3];Tang, Wei[2,4];Zhang, Shuang[3];Cao, Lingyan[3];Yuan, Yuan[1,2,3];Wang, Jing[1,2,3];Liu, Changsheng[1,2,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Minist Educ, Key Lab Ultrafine Mat, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Minist Educ, Engn Res Ctr Biomed Mat, Shanghai 200237, Peoples R China;[4]Chinese Acad Sci, Shenzhen Inst Adv Technol, Res Ctr Human Tissues & Organs Degenerat, Shenzhen 518055, Guangdong, Peoples R China

年份：2018

卷号：71

起止页码：510

外文期刊名：ACTA BIOMATERIALIA

收录：;EI(收录号：20215111350095);WOS:【SCI-EXPANDED(收录号:WOS:000431470300042)】；

基金：The authors wish to express their gratitude to the financial supports from National Natural Science Foundation of China (No. 31330028, No. 31470923), International Cooperation Project of Shanghai Science and Technique Committee (15520711100), Major Basic Research Foundation of Shanghai Science and Technique Committee (14JC1490800) and Key Project of Logistic Scientific Research of PLA (BWS13C014). This study is also supported by 111 Project (B14018).

语种：英文

外文关键词：Sulfated chitosan; Vascular endothelial growth factor; Affinity; Angiogenesis

摘要：Emerging evidence suggests that vascular endothelial growth factor (VEGF) is important in the treatment of various ischemic and cardiovascular diseases. However, it often suffers from high cost and easy deactivation with a short half-life. Here, we describe a synthetic 2-N, 6-O-sulfated chitosan (26SCS) with a high affinity to VEGF promoting the binding of the signaling protein to its VEGF receptor 2 (VEGFR2), activating receptor phosphorylation and pro-angiogenic related genes expression, and further stimulating downstream VEGF-dependent endothelial cell viability, migration, tube formation and rat aortic rings outgrowth. Interestingly, the obvious recruitment of mural cells were occurred to stabilize the sprouted microvessels. In addition, the pro-angiogenic potential of 26SCS composited VEGF was confirmed in vivo using the chick embryo chorioallantoic membrane (CAM) assay with an extensive perfusable vascular network. A longer monitoring was administered subcutaneously to mice in a biocompatible gelatin sponge and showed that VEGF with 26SCS had the capability to efficiently enhance neovascularization. These findings highlight that 26SCS, the semi-synthetic natural polymer, may be a promising coagent with VEGF for vascular therapy.