文献类型：期刊文献

英文题名：Efficient degradation of sulfadiazine by photosynthetic cyanobacteria Synechocystis sp. PCC 6803 coupling with recombinant laccase strategy

作者：Wu, Ping[1,2];Hu, Tianyouzi[1,3];Sun, Liyun[1,2];Fan, Jianhua[1,2,4,5]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Dept Appl Biol, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Dept Bioengn, Shanghai 200237, Peoples R China;[4]Shihezi Univ, Sch Chem & Chem Engn, Shihezi 832003, Peoples R China;[5]130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2023

卷号：474

外文期刊名：CHEMICAL ENGINEERING JOURNAL

收录：;EI(收录号：20233714725363);WOS:【SCI-EXPANDED(收录号:WOS:001080869800001)】；

基金：This work was sponsored by National Key Research and Development Project of China 2020YFA0907304 and 2019YFA0906300, Natural Science Foundation of Shandong Province ZR2019ZD17, Natural Science Foundation of Shanghai 23ZR1415100 and 21ZR1416400, and Funding Project of the State Key Laboratory of Bioreactor Engineering and Shanghai Collaborative Innovation Center for Biomanufacturing Technology.

语种：英文

外文关键词：Synechocystis; Sulfadiazine; Bioremediation; Laccase; Microalgae

摘要：As an emerging technology, photosynthetic algae have great potential for bioremediation of various organic pollutants in wastewater. However, the application of recombinant enzyme strategy in algae bioremediation is still in its infancy. In this study, the removal effect of sulfadiazine (SDZ) by Synechocystis sp. PCC 6803 was thoroughly explored. The removal rate of 0.5-2 mg/L SDZ reached more than 85% within 60 h, which is the highest level reported in algae. Biodegradation was the primary mechanism, with similar to 24.5% SDZ removed by combining photolysis and hydrolysis. In-depth analysis of the transformation pathway confirmed that laccase was the main enzymatic contributor to biodegradation. The medium syringic acid was added to form a laccase-mediator system, and the removal rate was increased by 101%. Furthermore, the recombinant laccase strategy was innovatively adopted to introduce efficiency heterogeneous laccase. A combination test was conducted on three modules (neutral site, promoter, and chaperone protein), and the optimal transformant increased the removal rate by 62%. In a word, this work elucidates the biodegradation pathway of SDZ by Synechocystis sp. in detail, reveals the critical role of laccase in algae bioremediation, which hope to accelerate the green and sustainable bioremediation process of antibiotics even a series of organic pollutants.