文献类型：期刊文献

英文题名：Application of a bilayer tubular scaffold based on electrospun poly(L-lactide-co-caprolactone)/collagen fibers and yarns for tracheal tissue engineering

作者：Wu, Tong[1];Zheng, Hui[2];Chen, Jianfeng[3];Wang, Yuanfei[4];Sun, Binbin[1];Morsi, Yosry[5];El-Hamshary, Hany[6,7];Al-Deyab, Salem S.[6];Chen, Chang[2];Mo, Xiumei[1]

机构：[1]Donghua Univ, State Key Lab Modificat Chem Fibers & Polymer Mat, Coll Chem Chem Engn & Biotechnol, 2999 North Renmin Rd, Shanghai 201620, Peoples R China;[2]Tongji Univ, Affiliated Shanghai Pulm Hosp, Shanghai 200433, Peoples R China;[3]Donghua Univ, Coll Mat Sci & Engn, Shanghai 201620, Peoples R China;[4]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Sch Resources & Environm Engn, Shanghai 200237, Peoples R China;[5]Swinburne Univ Technol, Fac Engn & Ind Sci, Hawthorn, Vic 3122, Australia;[6]King Saud Univ, Dept Chem, Coll Sci, Riyadh 11451, Saudi Arabia;[7]Tanta Univ, Fac Sci, Dept Chem, Tanta 31527, Egypt

年份：2017

卷号：5

期号：1

起止页码：139

外文期刊名：JOURNAL OF MATERIALS CHEMISTRY B

收录：;EI(收录号：20165203195790);WOS:【SCI-EXPANDED(收录号:WOS:000392417300012)】；

基金：This research was supported by the National Natural Science Foundation of China (31470941 and 31271035), the Science and Technology Commission of Shanghai Municipality (15JC1490100 and 15441905100), the National Major Research Program of China (2016YFC1100200), the PhD Programs Foundation of Ministry of Education of China (20130075110005) and the light of textile project (J201404), The authors extend their appreciation to the International Scientific Partnership Program ISPP at King Saud University for its funding research through (ISPP# 0049).

语种：英文

外文关键词：Cells - Electrospinning - Scaffolds (biology) - Tissue - Body fluids - Collagen - Porosity - Rats - Respiratory system - Biocompatibility - Tissue regeneration

摘要：A bilayer tubular scaffold (BLTS) consisting of poly(L-lactide-co-caprolactone) (P(LLA-CL))/collagen submicron sized fibers and micron sized yarns, was prepared via electrospinning. Then, autologous tracheal epithelial cells and chondrocytes were separately seeded onto the two layers of the BLTS. After culturing for 7 days, the cell-seeded BLTS (CS-BLTS) was implanted and wrapped in rat tracheal fascia for pre-vascularization. The pre-vascularized BLTS (PV-BLTS) was subjected to an in situ trachea regeneration study using a rat trachea injury model, along with CS-BLTS and bare BLTS for comparison. The results presented the bilayer structure of the BLTS, and the two layers were arranged conterminously. The porosity of the outer layer (collagen/P(LLA-CL) yarns) was found to be significantly higher (P < 0.05) than that of the inner layer (collagen/P(LLA-CL) fibers). In vitro biological analysis demonstrated that the collagen/P(LLA-CL) showed good biocompatibility, which promoted tracheal epithelial cell initial adhesion and proliferation with a highly significant difference (P < 0.001) or significant difference (P < 0.05) compared to those of pure P(LLA-CL) materials respectively. Chondrocyte activity and proliferation were also enhanced on collagen/P(LLA-CL) yarns with a significant difference (P < 0.05) compared to those of pure P(LLA-CL). Chondrocyte penetration was promoted as well, due to the loose and porous structure of the electrospun collagen/P(LLA-CL) yarns. The in vivo evaluation results of immune response analysis and histological investigation demonstrated that the PV-BLTS performed better in new capillary regeneration, reducing immunogenicity and improving tracheal tissue regeneration compared to the CS-BLTS and bare BLTS, indicating its promising potential as a new tissue engineered alternative for trachea repair and regeneration.