文献类型：期刊文献

英文题名：Degradation of MXC by host/guest-type immobilized laccase on magnetic tubular mesoporous silica

作者：Yang, Yuxiang[1];Wei, Qinmei[1];Zhang, Jianbo[2];Xi, Yanjie[1];Yuan, Hongming[3];Chen, Cheng[1];Liu, Xiangnong[4]

机构：[1]E China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[2]Peking Univ, Dept Environm Sci, Beijing 100871, Peoples R China;[3]Jilin Univ, Coll Chem, State Key Lab Inorgan Synth & Preparat Chem, Changchun 130012, Peoples R China;[4]Yangzhou Univ, Anal Test Ctr, Yangzhou 225009, Jiangsu, Peoples R China

年份：2015

卷号：97

起止页码：111

外文期刊名：BIOCHEMICAL ENGINEERING JOURNAL

收录：;EI(收录号：20150800551372);WOS:【SCI-EXPANDED(收录号:WOS:000353004100014)】；

基金：This work was supported by the National Natural Science Foundation of China (20577010, 20971043) and the Open Project Program of State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, Jilin University.

语种：英文

外文关键词：Tubular mesoporous SiO2; Immobilized enzymes; Biocatalysis; MXC; Kinetic parameters; Biodegradation

摘要：In this paper, magnetic host/guest-type immobilized laccase was prepared by co-adsorption of superparamagnetic particles (SPMNPs) and laccase into the pore channels of tubular mesoporous SiO2 by the "size-matching effect". After immobilization, the thermal stability and repetition usage-ratio of immobilized laccase were improved significantly, the loading amount of enzyme and SPMNPs was determined by Lowry's method and EDS (Energy Dispersive Spectroscopy), respectively. Under the optimal conditions, the removal efficiency of MXC (Methoxychlor) by immobilized laccase reached 69.4% and the removal efficiency still remained 45.4% after six cycles of operations. The studies on degradation kinetics of MXC by free and immobilized laccase were also carried out, respectively. In virtue of GC MS, H-1 NMR and C-13 NMR analysis, (CH3OC6H5)(2)C=CHCl and 1,1-diphenylethylene were identified as intermediate and final degradation products of MXC, respectively, further demonstrating the degradation mechanism of MXC by the immobilized laccase. (C) 2015 Elsevier B.V. All rights reserved.