文献类型：期刊文献

英文题名：Binding site profiles and N-terminal minor groove interactions of the master quorum-sensing regulator LuxR enable flexible control of gene activation and repression

作者：Zhang, Jun[1];Liu, Bing[5];Gu, Dan[6];Hao, Yuan[1];Chen, Mo[1];Ma, Yue[1];Zhou, Xiaohui[7];Reverter, David[5];Zhang, Yuanxing[1,3,4];Wang, Qiyao[1,2,4]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao, Peoples R China;[3]Southern Marine Sci & Engn Guangdong Lab Zhuhai, Zhuhai 519000, Peoples R China;[4]Shanghai Engn Res Ctr Maricultured Anim Vaccines, Shanghai, Peoples R China;[5]Univ Autonoma Barcelona, Inst Biotecnol & Biomed, Dept Bioquim & Biol Mol, Bellaterra 08193, Spain;[6]Yangzhou Univ, Jiangsu Coinnovat Ctr Prevent & Control Important, Jiangsu Key Lab Zoonosis, Yangzhou 225009, Jiangsu, Peoples R China;[7]Univ Connecticut, Dept Pathobiol & Vet Sci, Storrs, CT 06269 USA

年份：2021

卷号：49

期号：6

起止页码：3274

外文期刊名：NUCLEIC ACIDS RESEARCH

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000642330200024)】；

基金：National Natural Science Foundation of China [31772891 to Q.W., 31772893 to Y.M.]; Ministry of Agriculture of China [CARS-47-G17]; Science and Technology Commission of Shanghai Municipality [17391902000]; Fundamental Research Funds for the Central Universities [22221062017019]; Ministerio de Ciencia, Innovacion y Universidades [PGC2018-098423-B-I00 to D.R.]. Funding for open access charge: National Natural Science Foundation of China.

语种：英文

摘要：LuxR is a TetR family master quorum sensing (QS) regulator activating or repressing expression of hundreds of genes that control collective behaviors in Vibrios with underlying mechanism unknown. To illuminate how this regulator controls expression of various target genes, we applied ChIP-seq and DNase I-seq technologies. Vibrio alginolyticus LuxR controls expression of similar to 280 genes that contain either symmetric palindrome (repDNA) or asymmetric (actDNA) binding motifs with different binding profiles. The median number of LuxR binding sites for activated genes are nearly double for that of repressed genes. Crystal structures of LuxR in complex with the respective repDNA and actDNA motifs revealed a new mode of LuxR DNA binding that involves contacts of its N-terminal extension to the minor groove. The N-terminal contacts mediated by Arginine-9 and Arginine-11 differ when LuxR binds to repDNA vs actDNA, leading to higher binding affinity at repressed targets. Moreover, modification of LuxR binding sites, binding profiles, and N-terminal extension have important consequences on QS-regulated phenotypes. These results facilitate fundamental understanding of the high flexibility of mechanisms of LuxR control of gene activation and repression in Vibrio QS, which may facilitate to design QS inhibiting chemicals that interfere with LuxR regulation to effectively control pathogens.