文献类型：期刊文献

英文题名：Inhibitory effect of chitooligosaccharides on retinol metabolism and bioavailability in mice

作者：Li, Wei[1,2];Zhao, Mengyao[1,2];Qin, Zhen[1,2];Chen, Qiming[1,2];Fan, Liqiang[1,2];Zhou, Jiachun[1,2];Zhao, Liming[1,2,3]

机构：[1]East China Univ Sci & Technol, Sch Biotechnol, State Key Lab Bioreactor Engn, R&D Ctr Separat & Extract Technol Fermentat Ind, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]SCICBT, Shanghai, Peoples R China;[3]Huizhou Long Dragon Biotechnol Co Ltd, Huizhou, Peoples R China

年份：2019

卷号：43

期号：5

外文期刊名：JOURNAL OF FOOD BIOCHEMISTRY

收录：;EI(收录号：20191206647785);WOS:【SCI-EXPANDED(收录号:WOS:000468395500014)】；

基金：The National Key Research and Development Program of China, Grant/Award Number: 2017YFB0309302; The "Shu Guang" project of the Shanghai Municipal Education Commission and the Shanghai Education Development Foundation, Grant/Award Number: 15SG28; National Natural Science Foundation of China, Grant/Award Number: No. 31371725

语种：英文

外文关键词：chemical interaction; chitooligosaccharides; retinol; retinol bioavailability; retinol metabolism

摘要：This study investigated the intervention effects of chitooligosaccharides (COS) on retinol metabolism and included comparisons of the retinol level, retinol binding protein 4 (RBP4) content, key genes, and protein expression between mice on a COS-enriched diet and a normal diet. The results showed that COS markedly decreased the retinol and RBP4 concentrations in the serum and liver. Furthermore, COS suppressed the mRNA and protein expression of RBP4, cellular retinol binding protein 1 (CRBP1), lecithin: retinol acyltransferase (LRAT) and cytochrome P45026A1 (CYP26A1). In addition, COS inhibited the mRNA expression of stimulated by retinoic acid 6 (STRA6). However, the protein expression of STRA6 was not significantly decreased. Thus, COS reduced the retinol concentration in the serum and disrupted the metabolism of retinol. The intervention mechanism of COS on retinol metabolism may be attributed to the modulation of RBP4, CRBP1, LRAT, STRA6, and CYP26A1 expression at the mRNA and protein levels. Practical applicationsChitooligosaccharides (COS), known to be the degradation products of chitosan, have been found to induce pinkeye in industrial workers who participate in the manufacturing of COS. Meanwhile, 5% population with COS dietary supplement also have similar phenomenon. The aim of this study is to explore the possible mechanism underlay of this potential risk. The results of this study showed that high exposure to COS during manufacture influences retinol metabolism and leads to a decrease in retinol content, ultimately causing pinkeye. These findings provide new evidence for understanding COS-induced retinol metabolism alteration and drawing attention toward the prevention of potential risk in high-exposure populations.